Prolactin Protein, Human (SF9, His)

Prolactin Protein acts on the mammary gland, promoting lactation through its interaction with the receptor PRLR. Prolactin Protein, Human (SF9, His) is the recombinant human-derived Prolactin protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Prolactin Protein, Human (SF9, His) is 199 a.a., with molecular weight of ~24.3 kDa.

Prolactin is a protein that primarily acts on the mammary gland by promoting lactation. It achieves this by interacting with its receptor, PRLR.

1.Measured by its ability to promote proliferation of INS-1 cells and the ED₅₀ is typically 5.6 ng/mL.
2.Measured in a cell proliferation assay using Nb2-11 rat lymphoma cells. The ED₅₀ for this effect is 0.8715 ng/mL, corresponding to a specific activity is 1.147×10⁶ U/mg.

Human

Sf9 insect cells

C-His

P01236 (L29-C227)

5617  [NCBI]

LPICPGGAARCQVTLRDLFDRAVVLSHYIHNLSSEMFSEFDKRYTHGRGFITKAINSCHTSSLATPEDKEQAQQMNQKDFLSLIVSILRSWNEPLYHLVTEVRGMQEAPEAILSKAVEIEEQTKRLLEGMELIVSQVHPETKENEIYPVWSGLPSLQMADEESRLSAYYNLLHCLRRDSHKIDNYLKLLKCRIIHNNNC

Approximately 24.3-25 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% Glycerol, pH 8.0 or 20 mM Tris-HCL, 500 mM NaCl, pH 8.0, 20% Glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.