Prolactin Protein, Mouse (Sf9, His)

Prolactin proteins exhibit hormone and receptor binding activities. It regulates mammary gland development, maternal behavior, and JAK-STAT signaling. Prolactin Protein, Mouse (Sf9, His) is the recombinant mouse-derived Prolactin protein, expressed by Sf9 insect cells , with C-His labeled tag.

Prolactin, a versatile protein, is predicted to exhibit hormone activity and prolactin receptor binding activity. It plays a pivotal role upstream of various processes, including mammary gland development, maternal behavior, and positive regulation of the receptor signaling pathway via JAK-STAT. Found in secretory granules, prolactin demonstrates expression in key structures such as the hippocampus, pituitary gland, placenta, and trophoblast giant cell. The human ortholog of this gene, PRL (prolactin), has implications in carotid artery disease, suggesting its potential involvement in physiological and pathological contexts. Investigations into prolactin function may contribute to a deeper understanding of its diverse roles and its impact on health.

1.Measured by its ability to promote proliferation of INS-1 cells and the ED₅₀ is typically 50-500 ng/mL.
2.Measured by its ability to induce PRL pathway activation in a Luciferase receptor Assay System. The ED50 for this effect is typically 50-500 ng/mL.

Mouse

Sf9 insect cells

C-His

NP_035294.2 (L32-C228)

19109  [NCBI]

Approximately 23-26 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10% glycerol or pH 7.5, 10% Glycerol, 300 mM NaCl, 20 mM Tris.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.