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  4. ST2/IL1RL1 Protein, Human (HEK293, Fc)

ST2/IL1RL1 Protein, Human (HEK293, Fc)

Cat. No.: HY-P76091
SDS COA Handling Instructions

ST2/IL1RL1 Protein potently inhibits IL-33 signaling. ST2/IL1RL1 Protein, Human (HEK293, Fc) is the recombinant human-derived ST2/IL1RL1 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of ST2/IL1RL1 Protein, Human (HEK293, Fc) is 310 a.a., with molecular weight of 75-100 kDa.

For research use only. We do not sell to patients.

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5 μg $49 In-stock
10 μg $84 In-stock
50 μg $235 In-stock
100 μg $400 In-stock
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  • Biological Activity

  • Technical Parameters

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Description

ST2/IL1RL1 Protein potently inhibits IL-33 signaling. ST2/IL1RL1 Protein, Human (HEK293, Fc) is the recombinant human-derived ST2/IL1RL1 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of ST2/IL1RL1 Protein, Human (HEK293, Fc) is 310 a.a., with molecular weight of 75-100 kDa.

Background

ST2/IL1RL1 protein serves as a potent inhibitor of IL-33 signaling.

Biological Activity

Immobilized Human IL-33, No Tag at 2 μg/mL (100 μl/well) on the plate. Dose response curve for Human IL-1RL1, hFc Tag with the EC50 of ≤9.9 ng/mL determined by ELISA.

Species

Human

Source

HEK293

Tag

C-hFc

Accession

Q01638-2 (K19-F328)

Gene ID
Molecular Construction
N-term
ST2 (K19-F328)
Accession # Q01638-2
hFc
C-term
Synonyms
Interleukin-1 receptor-like 1; Protein ST2; IL1RL1; DER4; ST2; T1
Molecular Weight

75-100 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE or Tris-Bis PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

ST2/IL1RL1 Protein, Human (HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ST2/IL1RL1 Protein, Human (HEK293, Fc)
Cat. No.:
HY-P76091
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