TNKS1 Protein, Human (His)

The TNKS1 protein is a polyADP-ribosyltransferase that is integral to multiple cellular processes, including the Wnt signaling pathway, telomere length regulation, and vesicle trafficking. In Wnt signaling, TNKS1 activates this pathway by poly-ADP-ribosylating AXIN1 and AXIN2, promoting their degradation. TNKS1 Protein, Human (His) is the recombinant human-derived TNKS1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of TNKS1 Protein, Human (His) is 235 a.a., .

TNKS1, a poly-ADP-ribosyltransferase, plays a pivotal role in diverse cellular processes, including the Wnt signaling pathway, telomere length regulation, and vesicle trafficking. In the Wnt signaling cascade, TNKS1 acts as an activator by mediating poly-ADP-ribosylation (PARsylation) of key components of the beta-catenin destruction complex, namely AXIN1 and AXIN2. This modification facilitates their recognition by RNF146, leading to ubiquitination and subsequent degradation. TNKS1 is also involved in the regulation of telomere length through PARsylation of TERF1. Furthermore, it participates in centrosome maturation during prometaphase by mediating PARsylation of HEPACAM2/MIKI and may influence vesicle trafficking by modulating the subcellular distribution of SLC2A4/GLUT4-vesicles. Additionally, TNKS1 is implicated in spindle pole assembly through PARsylation of NUMA1 and contributes to the stimulation of 26S proteasome activity, highlighting its multifaceted functions in cellular physiology.

Human

E. coli

N-6*His

O95271 (Q1091-Q1325)

8658

28.8 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, 200 mM NaCl, 20% glycerol, 1 mM DTT, pH 7.5.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.