TrkA Protein, Human (HEK293)

TrkA Protein, Human (HEK293) is the high affinity catalytic receptor for the neurotrophin, nerve growth factor (NGF), and mediates the multiple effects of NGF, which include neuronal differentiation and avoidance of programmed cell death.

Tyrosine kinase receptor A (TrkA) is the high affinity catalytic receptor for the neurotrophin, nerve growth factor (NGF), and mediates the multiple effects of NGF, which include neuronal differentiation and avoidance of programmed cell death. TrkA is originally cloned from a colon tumor; the cancer occurred via a translocation, which results in the activation of the TrkA kinase domain^[1][2].

The ED₅₀ is <1 μg/mL as measured by a neutralization assay in the presence of 10.0 ng/ml human β-NGF using TF-1 Cells.

Human

HEK293

Tag Free

P04629-1 (A34-P407)

4914  [NCBI]

APCPDACCPHGSSGLRCTRDGALDSLHHLPGAENLTELYIENQQHLQHLELRDLRGLGELRNLTIVKSGLRFVAPDAFHFTPRLSRLNLSFNALESLSWKTVQGLSLQELVLSGNPLHCSCALRWLQRWEEEGLGGVPEQKLQCHGQGPLAHMPNASCGVPTLKVQVPNASVDVGDDVLLRCQVEGRGLEQAGWILTELEQSATVMKSGGLPSLGLTLANVTSDLNRKNVTC

65-85 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized after extensive dialysis against PBS.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Benito-Gutiérrez E, et al. Origin and evolution of the Trk family of neurotrophic receptors. Mol Cell Neurosci. 2006 Feb;31(2):179-92.  [Content Brief]

  [2]. Lambiase A, et al. Molecular basis for keratoconus: lack of TrkA expression and its transcriptional repression by Sp3. Proc Natl Acad Sci U S A. 2005 Nov 15;102(46):16795-800.  [Content Brief]