TYRO3/DTK Protein, Human (388a.a, HEK293, His)

TYRO3/DTK protein is a receptor tyrosine kinase that transduces signals by binding to ligands such as TULP1 or GAS6. TYRO3 is activated by ligand binding and regulates cell survival, migration, and differentiation, leading to dimerization and intracellular autophosphorylation to create docking sites for downstream signaling molecules. TYRO3/DTK Protein, Human (388a.a, HEK293, His) is the recombinant human-derived TYRO3/DTK protein, expressed by HEK293 , with C-6*His labeled tag. The total length of TYRO3/DTK Protein, Human (388a.a, HEK293, His) is 388 a.a., with molecular weight of 55-70 kDa.

The TYRO3/DTK protein is a receptor tyrosine kinase that transmits signals from the extracellular matrix to the cytoplasm by binding to ligands such as TULP1 or GAS6. It regulates various physiological processes including cell survival, migration, and differentiation. When ligands bind to TYRO3 at the cell surface, it leads to dimerization and autophosphorylation of its intracellular domain, creating docking sites for downstream signaling molecules. This activation also interacts with PIK3R1, enhancing PI3-kinase activity and activating the AKT survival pathway. This pathway includes nuclear translocation of NF-kappa-B and up-regulation of NF-kappa-B-regulated genes. TYRO3 signaling is involved in protecting neurons from excitotoxic injury, promoting platelet aggregation, and reorganizing the cytoskeleton. Additionally, TYRO3 plays a crucial role in inhibiting the innate immune response triggered by Toll-like receptors (TLRs) by activating STAT1, which selectively induces the production of suppressors of cytokine signaling SOCS1 and SOCS3. It is also noteworthy that TYRO3 functions as a receptor for lassa virus and lymphocytic choriomeningitis virus, potentially through GAS6 binding to phosphatidyl-serine on the surface of the virion envelope.

Human

HEK293

C-6*His

Q06418 (A41-S428)

7301  [NCBI]

AGLKLMGAPVKLTVSQGQPVKLNCSVEGMEEPDIQWVKDGAVVQNLDQLYIPVSEQHWIGFLSLKSVERSDAGRYWCQVEDGGETEISQPVWLTVEGVPFFTVEPKDLAVPPNAPFQLSCEAVGPPEPVTIVWWRGTTKIGGPAPSPSVLNVTGVTQSTMFSCEAHNLKGLASSRTATVHLQALPAAPFNITVTKLSSSNASVAWMPGADGRALLQSCTVQVTQAPGGWEVLAVVVPVPPFTCLLRDLVPATNYSLRVRCANALGPSPYADWVPFQTKGLAPASAPQNLHAIRTDSGLILEWEEVIPEAPLEGPLGPYKLSWVQDNGTQDELTVEGTRANLTGWDPQKDLIVRVCVSNAVGCGPWSQPLVVSSHDRAGQQGPPHSRTS

55-70 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.