RH-5849

Cat. No.: HY-118541 Purity: 99.35%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

RH-5849 is an orally active agonist for non-steroidal ecdysteroid, which inhibits the larval growth of Plodia inferpuncteila in a dose-dependent manner. RH-5849 exhibits molting hormone activity.

For research use only. We do not sell to patients.

RH-5849 Chemical Structure

CAS No. : 112225-87-3

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
250 mg	In-stock	Estimated Time of Arrival: December 31
1 g	Get quote	Estimated Time of Arrival: December 31
5 g	Get quote	Estimated Time of Arrival: December 31
10 g	Get quote
50 g	Get quote

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Description

In Vitro

RH-5849 (0-100 μM, 3 days) inhibits proliferation of IAL-PID2 cells in a dose-dependent manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

RH-5849 (0-50 ppm, po for 17 days) induces molts and cuticle formation by wing discs, inhibits larval growth and results in death in a dose-dependent manner in Plodia inferpuncteila model^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

296.36

Formula

C₁₈H₂₀N₂O₂

CAS No.

112225-87-3

Appearance

Solid

Color

White to off-white

SMILES

CC(C)(C)N(NC(C1=CC=CC=C1)=O)C(C2=CC=CC=C2)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 200 mg/mL (674.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.3743 mL	16.8714 mL	33.7427 mL
5 mM	0.6749 mL	3.3743 mL	6.7485 mL
10 mM	0.3374 mL	1.6871 mL	3.3743 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (16.87 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (16.87 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.35%

Select Batch:

Data Sheet (272 KB) SDS (392 KB)

COA (248 KB) HNMR (243 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

References

[1]. Silhacek D L, et al., Action of RH 5849, a non‐steroidal ecdysteroid mimic, on Plodia interpunctella (Hübner) in vivo and in vitro[J]. Archives of Insect Biochemistry and Physiology, 1990, 15(4): 201-212.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.3743 mL	16.8714 mL	33.7427 mL	84.3569 mL
	5 mM	0.6749 mL	3.3743 mL	6.7485 mL	16.8714 mL
	10 mM	0.3374 mL	1.6871 mL	3.3743 mL	8.4357 mL
	15 mM	0.2250 mL	1.1248 mL	2.2495 mL	5.6238 mL
	20 mM	0.1687 mL	0.8436 mL	1.6871 mL	4.2178 mL
	25 mM	0.1350 mL	0.6749 mL	1.3497 mL	3.3743 mL
	30 mM	0.1125 mL	0.5624 mL	1.1248 mL	2.8119 mL
	40 mM	0.0844 mL	0.4218 mL	0.8436 mL	2.1089 mL
	50 mM	0.0675 mL	0.3374 mL	0.6749 mL	1.6871 mL
	60 mM	0.0562 mL	0.2812 mL	0.5624 mL	1.4059 mL
	80 mM	0.0422 mL	0.2109 mL	0.4218 mL	1.0545 mL
	100 mM	0.0337 mL	0.1687 mL	0.3374 mL	0.8436 mL

RH-5849 Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

RH-5849112225-87-3RH5849RH 5849InsecticidePlodia inferpuncteilaIAL-PID2Inhibitorinhibitorinhibit

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RH-5849

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