Rhodamine dithenoyl hydrazide

Name: Rhodamine dithenoyl hydrazide
Brand: MedChemExpress
SKU: HY-158739
Rating: 4.5 (1 reviews)

Cat. No.: HY-158739 Purity: 98.00%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

Rhodamine dithenoyl hydrazide possesses excellent recognition and selectivity for Fe³⁺ ion. Rhodamine dithenoyl hydrazide exhibits Excitation/Emission maxima of 543/550-700 nm, respectively.

For research use only. We do not sell to patients.

Rhodamine dithenoyl hydrazide Chemical Structure

CAS No. : 2740257-94-5

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	Get quote	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Rhodamine dithenoyl hydrazide possesses excellent recognition and selectivity for Fe³⁺ ion. Rhodamine dithenoyl hydrazide exhibits Excitation/Emission maxima of 543/550-700 nm, respectively^[1].

Molecular Weight

676.85

Formula

C₃₈H₃₆N₄O₄S₂

CAS No.

2740257-94-5

Appearance

Solid

Color

White to yellow

SMILES

O=C(N(N1C(C2=C(C13C4=CC=C(N(CC)CC)C=C4OC5=CC(N(CC)CC)=CC=C53)C=CC=C2)=O)C(C6=CC=CS6)=O)C7=CC=CS7

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (36.94 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.4774 mL	7.3872 mL	14.7743 mL
5 mM	0.2955 mL	1.4774 mL	2.9549 mL
10 mM	0.1477 mL	0.7387 mL	1.4774 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (3.69 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.00%

Select Batch:

Data Sheet (280 KB) SDS (252 KB)

COA (263 KB)

Handling Instructions (2659 KB)

References

[1]. Yi Liu, et al. A selective N,N-dithenoyl-rhodamine based fluorescent probe for Fe3+ detection in aqueous and living cells. J Environ Sci (China). 2020 Apr:90:180-188. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.4774 mL	7.3872 mL	14.7743 mL	36.9358 mL
	5 mM	0.2955 mL	1.4774 mL	2.9549 mL	7.3872 mL
	10 mM	0.1477 mL	0.7387 mL	1.4774 mL	3.6936 mL
	15 mM	0.0985 mL	0.4925 mL	0.9850 mL	2.4624 mL
	20 mM	0.0739 mL	0.3694 mL	0.7387 mL	1.8468 mL
	25 mM	0.0591 mL	0.2955 mL	0.5910 mL	1.4774 mL
	30 mM	0.0492 mL	0.2462 mL	0.4925 mL	1.2312 mL