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  3. Fluorescent Dye
  4. RhoNox-1

RhoNox-1 is a fluorescent probe for the specific detection of divalent iron ions, and when RhoNox-1 reacts with Fe2+. RhoNox-1 can generate an irreversible orange (red) fluorescent product (Ex/Em：540/575 nm) . FeRhoNox-1 can enter the cell well, suitable for the detection of Fe2+ in living cells, and tends to be localized in the Golgi apparatus.

For research use only. We do not sell to patients.

RhoNox-1 Chemical Structure

RhoNox-1 Chemical Structure

CAS No. : 1447815-38-4

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 303 In-stock
Solution
10 mM * 1 mL in DMSO USD 303 In-stock
Solid
5 mg USD 300 In-stock
10 mg USD 480 In-stock
25 mg USD 1006 In-stock
50 mg USD 1610 In-stock
100 mg USD 2580 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Based on 5 publication(s) in Google Scholar

RhoNox-1 Related Antibodies

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Description

RhoNox-1 is a fluorescent probe for the specific detection of divalent iron ions, and when RhoNox-1 reacts with Fe2+. RhoNox-1 can generate an irreversible orange (red) fluorescent product (Ex/Em：540/575 nm) . FeRhoNox-1 can enter the cell well, suitable for the detection of Fe2+ in living cells, and tends to be localized in the Golgi apparatus[1].

In Vitro

1. Preparation of RhoNox-1 working solution
1.1 Preparation of the stock solution
Dissolve 50 μg RhoNox-1 in 110 μL DMSO to obtain 1 mM of stock solution.
1.2 Preparation of RhoNox-1 working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of working solution.
Note: Please adjust the concentration of RhoNox-1 working solution according to the actual situation.
2. Cell staining (6-well plate)
2.1 Suspension cells
a.Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL.
b.Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
c.Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
d.Wash twice with PBS, 5 minutes each time.
e.Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a.Culture adherent cells on sterile coverslips.
b.Remove the coverslip from the medium and aspirate excess medium.
c.Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes.
d.Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RhoNox-1 Related Antibodies
Molecular Weight

458.55

Formula

C28H30N2O4
CAS No.
Appearance

Solid

Color

Pink to red

Emission (Em)

575
Excitation (Ex)

540
SMILES

O=C(C1=C(C2=C3C=C/C(C=C3OC4=CC([N+](CC)(CC)[O-])=CC=C42)=[N+](CC)\CC)C=CC=C1)[O-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (218.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1808 mL 10.9039 mL 21.8079 mL
5 mM 0.4362 mL 2.1808 mL 4.3616 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1808 mL 10.9039 mL 21.8079 mL 54.5197 mL
5 mM 0.4362 mL 2.1808 mL 4.3616 mL 10.9039 mL
10 mM 0.2181 mL 1.0904 mL 2.1808 mL 5.4520 mL
15 mM 0.1454 mL 0.7269 mL 1.4539 mL 3.6346 mL
20 mM 0.1090 mL 0.5452 mL 1.0904 mL 2.7260 mL
25 mM 0.0872 mL 0.4362 mL 0.8723 mL 2.1808 mL
30 mM 0.0727 mL 0.3635 mL 0.7269 mL 1.8173 mL
40 mM 0.0545 mL 0.2726 mL 0.5452 mL 1.3630 mL
50 mM 0.0436 mL 0.2181 mL 0.4362 mL 1.0904 mL
60 mM 0.0363 mL 0.1817 mL 0.3635 mL 0.9087 mL
80 mM 0.0273 mL 0.1363 mL 0.2726 mL 0.6815 mL
100 mM 0.0218 mL 0.1090 mL 0.2181 mL 0.5452 mL
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RhoNox-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

RhoNox-11447815-38-4RhoNox1RhoNox 1Fluorescent Dyefluorescent probesLIPMMNK1HepG2 cellsiron metabolismInhibitorinhibitorinhibit

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