2. GPCR/G Protein Neuronal Signaling
  3. 5-HT Receptor
  4. Ro60-0175

Ro60-0175 is a potent and selective agonist of 5-HT2C receptor. Ro60-0175 reduces self-administration.

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Ro60-0175 Chemical Structure

Ro60-0175 Chemical Structure

CAS No. : 169675-08-5

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5 mg USD 280 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Ro60-0175:

Ro60-0175 Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Ro60-0175 is a potent and selective agonist of 5-HT2C receptor. Ro60-0175 reduces self-administration[1].

IC50 & Target

5-HT2C Receptor

 

In Vivo

Ro60-0175 (1 mg/kg; s.c.) preserves the regularity of responding seen in control animals in drug-treated group, but drug-treated animals reach their break-points earlier[1].
Ro60-0175 (0.3, 1, and 3 mg/kg; s.c.) significantly reduces responding on the active lever in the reinstatement group[1].
Ro60-0175 (0.5 mg/kg SB242084; 1 mg/kg Ro60-0175; s.c.; i.p.) reduces responding compared to vehicle in the reinstatement group, and that this effect is prevented by pretreatment with SB242084. For responding on the inactive lever, there are no significant main effects or interactions[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Adult male Sprague-Dawley rats (280-320 g)[1]
Dosage: 1 mg/kg
Administration: s.c.
Result: Preserved the regularity of responding seen in control animals in drug-treated group, but drug-treated animals reached their break-points earlier.
Animal Model: Adult male Sprague-Dawley rats (280-320 g)[1]
Dosage: 0.5 mg/kg (SB242084), 1 mg/kg (Ro60-0175)
Administration: s.c. (Ro60-0175), i.p. (SB242084)
Result: Reduced responding for cocaine and effect was reversed by SB242084.
Animal Model: Adult male Sprague-Dawley rats (280-320 g)[1]
Dosage: 0.3, 1, and 3 mg/kg (Ro60-0175), 1 mg/kg (yohimbine)
Administration: s.c. (Ro60-0175), i.p. (yohimbine)
Result: Showed yohimbine treatment alone increased responding relative to vehicle injection, and the response was attenuated dose dependently by Ro60-0175.
Animal Model: Adult male Sprague-Dawley rats (280-320 g)[1]
Dosage: 0.5 mg/kg (SB242084), 1 mg/kg (Ro60-0175), 1 mg/kg (yohimbine)
Administration: s.c. (Ro60-0175), i.p. (yohimbine), i.p. (SB242084)
Result: Ro60-0175 reduced responding and that this effect was prevented by SB242084 pretreatment.
Molecular Weight

226.68

Formula

C11H12ClFN2
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

N[C@@H](C)CN1C=CC2=C1C=C(Cl)C(F)=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (551.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.4115 mL 22.0575 mL 44.1151 mL
5 mM 0.8823 mL 4.4115 mL 8.8230 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (9.18 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (9.18 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.98%

COA (248 KB) HNMR (226 KB) LCMS (265 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.4115 mL 22.0575 mL 44.1151 mL 110.2876 mL
5 mM 0.8823 mL 4.4115 mL 8.8230 mL 22.0575 mL
10 mM 0.4412 mL 2.2058 mL 4.4115 mL 11.0288 mL
15 mM 0.2941 mL 1.4705 mL 2.9410 mL 7.3525 mL
20 mM 0.2206 mL 1.1029 mL 2.2058 mL 5.5144 mL
25 mM 0.1765 mL 0.8823 mL 1.7646 mL 4.4115 mL
30 mM 0.1471 mL 0.7353 mL 1.4705 mL 3.6763 mL
40 mM 0.1103 mL 0.5514 mL 1.1029 mL 2.7572 mL
50 mM 0.0882 mL 0.4412 mL 0.8823 mL 2.2058 mL
60 mM 0.0735 mL 0.3676 mL 0.7353 mL 1.8381 mL
80 mM 0.0551 mL 0.2757 mL 0.5514 mL 1.3786 mL
100 mM 0.0441 mL 0.2206 mL 0.4412 mL 1.1029 mL
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Ro60-0175 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ro60-0175169675-08-55-HT ReceptorSerotonin Receptor5-hydroxytryptamine Receptorselective5-HT2Cself-administrationdrug-seekingyohimbinecontextual cuesInhibitorinhibitorinhibit

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