2. Stem Cell/Wnt
  3. Hedgehog
  4. Robotnikinin

Robotnikinin is a small molecule capable of binding to and inhibiting the activity of Sonic Hedgehog (Shh) signaling up stream of Smo.

For research use only. We do not sell to patients.

Robotnikinin Chemical Structure

Robotnikinin Chemical Structure

CAS No. : 1132653-79-2

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1 mg USD 340 In-stock
5 mg USD 850 In-stock
10 mg USD 1360 In-stock
25 mg USD 2720 In-stock
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Based on 3 publication(s) in Google Scholar

Robotnikinin Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

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Description

Robotnikinin is a small molecule capable of binding to and inhibiting the activity of Sonic Hedgehog (Shh) signaling up stream of Smo[1][2].

In Vitro

Robotnikinin demonstrates ShhN-binding capacity at concentrations between 1.56 μM and 25 μM, with a KD of 3.1 μM derived from kinetic data[2].
Robotnikinin (50 µM) prevents the ShhN-induced LC3-II increase[3].
Robotnikinin (5 μM) downregulates classical NF-κB pathway proteins in H929 and U266 cell lines cocultured with HS-5 cells, suggesting a link between Hh signaling and the NF-κB pathway in MM[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Robotnikinin Related Antibodies

RT-PCR[4]

Cell Line: NCI-H929 or U266 cells.
Concentration: 5 μM.
Incubation Time: 48 h.
Result: Downregulated classical NF-κB pathway proteins in H929 and U266 cell lines cocultured with HS-5 cells.
Molecular Weight

454.95

Formula

C25H27ClN2O4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(NCC1=CC=C(Cl)C=C1)C[C@H](C/C=C/CC2)C(NC[C@@H](C3=CC=CC=C3)OC2=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 20 mg/mL (43.96 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1980 mL 10.9902 mL 21.9804 mL
5 mM 0.4396 mL 2.1980 mL 4.3961 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1980 mL 10.9902 mL 21.9804 mL 54.9511 mL
5 mM 0.4396 mL 2.1980 mL 4.3961 mL 10.9902 mL
10 mM 0.2198 mL 1.0990 mL 2.1980 mL 5.4951 mL
15 mM 0.1465 mL 0.7327 mL 1.4654 mL 3.6634 mL
20 mM 0.1099 mL 0.5495 mL 1.0990 mL 2.7476 mL
25 mM 0.0879 mL 0.4396 mL 0.8792 mL 2.1980 mL
30 mM 0.0733 mL 0.3663 mL 0.7327 mL 1.8317 mL
40 mM 0.0550 mL 0.2748 mL 0.5495 mL 1.3738 mL
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Robotnikinin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Robotnikinin1132653-79-2HedgehogSonicShhLC3-IIH929U266Inhibitorinhibitorinhibit

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