RUVBL1/2 ATPase-IN-1

Name: RUVBL1/2 ATPase-IN-1
Brand: MedChemExpress
SKU: HY-146074
Rating: 4.5 (1 reviews)

Cat. No.: HY-146074 Purity: 99.63%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

RUVBL1/2 ATPase-IN-1 (compound 18) is a potent and selective inhibitor of RUVBL1/2 ATPase with IC₅₀ values of 6.0 and 7.7 μM, respectively. RUVBL1 and RUVBL2 are highly conserved AAA ATPases (ATPases Associated with various cellular Activities) and highly relevant to the progression of cancer. RUVBL1/2 ATPase-IN-1 has the potential for the research of cancer diseases.

For research use only. We do not sell to patients.

RUVBL1/2 ATPase-IN-1 Chemical Structure

CAS No. : 423128-55-6

Size	Price	Stock	Quantity
1 mg	USD 300	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 600	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 960	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 1728	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 2420	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 3380	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	15 μM Compound: 18	Anti-proliferative activity against human A549 assessed as cell growth inhibition measured after 48 hrs by Cell Titre Glo Luminescent Cell viability assay Anti-proliferative activity against human A549 assessed as cell growth inhibition measured after 48 hrs by Cell Titre Glo Luminescent Cell viability assay	[PMID: 35364523]
HCT-116	IC₅₀	11 μM Compound: 18	Anti-proliferative activity against human HCT-116 assessed as cell growth inhibition measured after 48 hrs by Cell Titre Glo Luminescent Cell viability assay Anti-proliferative activity against human HCT-116 assessed as cell growth inhibition measured after 48 hrs by Cell Titre Glo Luminescent Cell viability assay	[PMID: 35364523]
MDA-MB-231	IC₅₀	8.9 μM Compound: 18	Anti-proliferative activity against human MDA-MB-231 assessed as cell growth inhibition measured after 48 hrs by Cell Titre Glo Luminescent Cell viability assay Anti-proliferative activity against human MDA-MB-231 assessed as cell growth inhibition measured after 48 hrs by Cell Titre Glo Luminescent Cell viability assay	[PMID: 35364523]

Molecular Weight

507.55

Formula

C₂₈H₂₈F₃N₅O

CAS No.

423128-55-6

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=C2N=C(C)C(CC3=CC=C(C)C=C3)=C(C)N2N=C1)N4CCN(C5=CC=CC(C(F)(F)F)=C5)CC4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 10 mg/mL (19.70 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9702 mL	9.8512 mL	19.7025 mL
5 mM	0.3940 mL	1.9702 mL	3.9405 mL
10 mM	0.1970 mL	0.9851 mL	1.9702 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (1.97 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1 mg/mL (1.97 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.63%

Select Batch:

Data Sheet (270 KB) SDS (252 KB)

COA (248 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Zhang G, et al. Discovery of small-molecule inhibitors of RUVBL1/2 ATPase. Bioorg Med Chem. 2022;62:116726. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.9702 mL	9.8512 mL	19.7025 mL	49.2562 mL
	5 mM	0.3940 mL	1.9702 mL	3.9405 mL	9.8512 mL
	10 mM	0.1970 mL	0.9851 mL	1.9702 mL	4.9256 mL
	15 mM	0.1313 mL	0.6567 mL	1.3135 mL	3.2837 mL