2. MAPK/ERK Pathway Protein Tyrosine Kinase/RTK TGF-beta/Smad Epigenetics
  3. p38 MAPK Src PKC
  4. SB 220025

SB 220025 is a reversible, orally active, cell-permeable, ATP-competitive and selective human p38 MAPK inhibitor (IC50 = 60 nM). SB 220025 also inhibits p56Lck and PKC with IC50 values of 3.5 and 2.89 µM, respectively. SB 220025 inhibits the expression of IL-8 gene in response to globular adiponectin (gAd), reduces inflammatory cytokine production and inhibits angiogenesis. SB 220025 effectively prevents the progression of arthritis in a chronic inflammatory disease model and can be used in the study of inflammation.

For research use only. We do not sell to patients.

SB 220025 Chemical Structure

SB 220025 Chemical Structure

CAS No. : 165806-53-1

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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5 mg USD 965 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of SB 220025:

SB 220025 Related Antibodies

Top Publications Citing Use of Products

View All p38 MAPK Isoform Specific Products:

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p38 MAPK p38α p38β MAPK13 p38δ p38γ

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Lck Fyn Blk

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PKC PKCα PKCβ PKCγ PKCδ PKCε PKCη PKCζ PKCθ PKCμ PKC-ι ℩ PKCι

  • Biological Activity

  • Purity & Documentation

  • References

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Description

SB 220025 is a reversible, orally active, cell-permeable, ATP-competitive and selective human p38 MAPK inhibitor (IC50 = 60 nM). SB 220025 also inhibits p56Lck and PKC with IC50 values of 3.5 and 2.89 µM, respectively. SB 220025 inhibits the expression of IL-8 gene in response to globular adiponectin (gAd), reduces inflammatory cytokine production and inhibits angiogenesis. SB 220025 effectively prevents the progression of arthritis in a chronic inflammatory disease model and can be used in the study of inflammation[1][2].

IC50 & Target[1]

p38

60 nM (IC50)

p56-Lck

3.5 μM (IC50)

PKC

2.89 μM (IC50)

In Vitro

SB 220025 (20 μM; 6 h) markedly reduces IL-8 gene expression in response to globular adiponectin (gAd) in HUVEC cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SB 220025 Related Antibodies

RT-PCR[1]

Cell Line: HUVEC cells
Concentration: 20 μM
Incubation Time: 6 h
Result: Inhibited MCP-1 gene expression.
In Vivo

SB 220025 (3-50 mg/kg; p.o.; single) inhibits inflammatory cytokine production in vivo[2].
SB 220025 (5, 30, 50 mg/kg; i.p.; b.i.d.) inhibits angiogenesis in the murine air pouch granuloma model[2].
SB 220025 (30 mg/kg; p.o.; twice a day for 3, 5, 7 or 14 days) prevents the increase in angiogenesis that occurs after day 3 in murine air pouch angiogenesis model[2].
SB 220025 (50 mg/kg; p.o.; b.i.d.; 10 days) effectively blocks the progression of arthritis in a chronic inflammatory disease model[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Acute model of LPS-induced TNF-a expression[2].
Dosage: 3-50 mg/kg
Administration: Oral administration; single; 30 min before challenge with LPS.
Result: Dosedependently inhibited TNF-a production with an ED50 value of 7.5 mg/kg, and showed more than 80% inhibition when at 50 mg/kg.
Animal Model: Murine air pouch granuloma model[2].
Dosage: 5, 30, 50 mg/kg
Administration: Intraperitoneal injection; bisindie (bid, twice a day).
Result: Caused a dose-dependent reduction in angiogenesis.
Animal Model: Murine air pouch granuloma model[2].
Dosage: 30 mg/kg
Administration: Oral administration; twice a day from day 0 until removal of granuloma tissue at days 3, 5, 7 or 14.
Result: Did not affect the initial burst of angiogenesis but did prevent the increase in angiogenesis that occurs after day 3.
Molecular Weight

338.38

Formula

C18H19FN6
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

NC1=NC=CC(C2=C(C3=CC=C(F)C=C3)N=CN2C4CCNCC4)=N1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (147.76 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9553 mL 14.7763 mL 29.5526 mL
5 mM 0.5911 mL 2.9553 mL 5.9105 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

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In Vivo Dissolution Calculator
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
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Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.90%

SDS (480 KB)

COA (256 KB) HNMR (253 KB) LCMS (93 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9553 mL 14.7763 mL 29.5526 mL 73.8814 mL
5 mM 0.5911 mL 2.9553 mL 5.9105 mL 14.7763 mL
10 mM 0.2955 mL 1.4776 mL 2.9553 mL 7.3881 mL
15 mM 0.1970 mL 0.9851 mL 1.9702 mL 4.9254 mL
20 mM 0.1478 mL 0.7388 mL 1.4776 mL 3.6941 mL
25 mM 0.1182 mL 0.5911 mL 1.1821 mL 2.9553 mL
30 mM 0.0985 mL 0.4925 mL 0.9851 mL 2.4627 mL
40 mM 0.0739 mL 0.3694 mL 0.7388 mL 1.8470 mL
50 mM 0.0591 mL 0.2955 mL 0.5911 mL 1.4776 mL
60 mM 0.0493 mL 0.2463 mL 0.4925 mL 1.2314 mL
80 mM 0.0369 mL 0.1847 mL 0.3694 mL 0.9235 mL
100 mM 0.0296 mL 0.1478 mL 0.2955 mL 0.7388 mL
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SB 220025 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SB 220025165806-53-1SB220025SB-220025p38 MAPKSrcPKCProtein kinase CHUVEC cellsinflammationgAdIL-8p56-LckarthritisInhibitorinhibitorinhibit

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