SH-BC-893

Name: SH-BC-893
Brand: MedChemExpress
SKU: HY-124758
Rating: 4.5 (1 reviews)

Cat. No.: HY-124758 Purity: 99.34%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

SH-BC-893 is an orally active anti-neoplastic sphingolipid analog. SH-BC-893 also protects from ceramide-induced mitochondrial dysfunction and corrects diet-induced obesity. SH-BC-893 can be used for the research of cancer and obesity.

For research use only. We do not sell to patients.

SH-BC-893 Chemical Structure

CAS No. : 1841409-92-4

Size	Price	Stock	Quantity
1 mg	USD 580	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 1500	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 2400	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 4560	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Description

Cellular Effect

Cell Line	Type	Value	Description	References
FL5.12	IC₅₀	1.8 μM Compound: 3; SH-BC-893	Inhibition of protein phosphatase 2A in mouse FL5.12 cells assessed as reduction in cell viability incubated for 48 hrs by propidium iodide staining based flow cytometric analysis Inhibition of protein phosphatase 2A in mouse FL5.12 cells assessed as reduction in cell viability incubated for 48 hrs by propidium iodide staining based flow cytometric analysis	[PMID: 31383588]
FL5.12	IC₅₀	1.9 μM Compound: 4	Cytotoxicity against mouse FL5.12A cells after 48 hrs by DAPI staining-based flow cytometric analysis Cytotoxicity against mouse FL5.12A cells after 48 hrs by DAPI staining-based flow cytometric analysis	[PMID: 27475534]

In Vitro

SH-BC-893 starves cancer cells to death by down-regulating cell surface nutrient transporters and blocking lysosomal trafficking events^[1].
SH-BC-893 (5 μM; 3 h) protects from ceramide-induced mitochondrial network fragmentation by disrupting intracellular trafficking^[2].
SH-BC-893 (8.935 nM; 3 h) protects from ceramide-induced mitochondrial dysfunction^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	p53^flox/flox MEFs
Concentration:	5 μM
Incubation Time:	3 h
Result:	Blocked palmitate-induced recruitment of DRP1 to mitochondria without affecting DRP1 protein levels.

Immunofluorescence^[1]

Cell Line:	p53^flox/flox MEFs
Concentration:	5 μM
Incubation Time:	3 h
Result:	Blocked palmitate-induced recruitment of DRP1 to mitochondria.

In Vivo

SH-BC-893 (oral; 120 mg/kg; single) robustly and acutely blocks ceramide-induced mitochondrial dysfunction, correcting diet-induced obesity and its metabolic sequelae^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6J mice (Male)^[1]
Dosage:	120 mg/kg
Administration:	Oral; single
Result:	Blocked palmitate- and ceramide-induced mitochondrial fission, preserved mitochondrial function, and prevented ER stress. Normalized mitochondrial morphology in the livers and brains of HFD-fed mice, improved mitochondrial function in white adipose tissue, and corrected aberrant plasma leptin and adiponectin levels. Restored normal body weight, glucose disposal, and hepatic lipid levels in mice consuming a HFD.

Molecular Weight

325.92

Formula

C₁₉H₃₂ClNO

CAS No.

1841409-92-4

Appearance

Liquid

Color

Colorless to light yellow

SMILES

OC[C@H]1NCC[C@@H]1C2=CC=C(CCCCCCCC)C=C2.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (153.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.0682 mL	15.3412 mL	30.6824 mL
5 mM	0.6136 mL	3.0682 mL	6.1365 mL
10 mM	0.3068 mL	1.5341 mL	3.0682 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.67 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.67 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.67 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.34%

Select Batch:

Data Sheet (272 KB) SDS (251 KB)

COA (244 KB) HNMR (245 KB) LCMS (90 KB)

Handling Instructions (2659 KB)

References

[1]. Kubiniok, Peter et al. Dynamic Phosphoproteomics Uncovers Signaling Pathways Modulated by Anti-oncogenic Sphingolipid Analogs. Molecular & cellular proteomics : MCP vol. 18,3 (2019): 408-422. [Content Brief]

[2]. Jayashankar, Vaishali et al. Drug-like sphingolipid SH-BC-893 opposes ceramide-induced mitochondrial fission and corrects diet-induced obesity. EMBO molecular medicine vol. 13,8 (2021): e13086. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.0682 mL	15.3412 mL	30.6824 mL	76.7059 mL
	5 mM	0.6136 mL	3.0682 mL	6.1365 mL	15.3412 mL
	10 mM	0.3068 mL	1.5341 mL	3.0682 mL	7.6706 mL
	15 mM	0.2045 mL	1.0227 mL	2.0455 mL	5.1137 mL
	20 mM	0.1534 mL	0.7671 mL	1.5341 mL	3.8353 mL
	25 mM	0.1227 mL	0.6136 mL	1.2273 mL	3.0682 mL
	30 mM	0.1023 mL	0.5114 mL	1.0227 mL	2.5569 mL
	40 mM	0.0767 mL	0.3835 mL	0.7671 mL	1.9176 mL
	50 mM	0.0614 mL	0.3068 mL	0.6136 mL	1.5341 mL
	60 mM	0.0511 mL	0.2557 mL	0.5114 mL	1.2784 mL
	80 mM	0.0384 mL	0.1918 mL	0.3835 mL	0.9588 mL
	100 mM	0.0307 mL	0.1534 mL	0.3068 mL	0.7671 mL

SH-BC-893 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SH-BC-8931841409-92-4Mitochondrial Metabolismoralsphingolipid analogceramide-induced mitochondrial dysfunctiondiet-induced obesityInhibitorinhibitorinhibit

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SH-BC-893

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Complete Stock Solution Preparation Table

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