2. Metabolic Enzyme/Protease
  3. Mitochondrial Metabolism
  4. SH-BC-893

SH-BC-893 is an orally active anti-neoplastic sphingolipid analog. SH-BC-893 also protects from ceramide-induced mitochondrial dysfunction and corrects diet-induced obesity. SH-BC-893 can be used for the research of cancer and obesity.

For research use only. We do not sell to patients.

SH-BC-893 Chemical Structure

SH-BC-893 Chemical Structure

CAS No. : 1841409-92-4

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1 mg USD 580 In-stock
5 mg USD 1500 In-stock
10 mg USD 2400 In-stock
25 mg USD 4560 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

SH-BC-893 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

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Description

SH-BC-893 is an orally active anti-neoplastic sphingolipid analog. SH-BC-893 also protects from ceramide-induced mitochondrial dysfunction and corrects diet-induced obesity. SH-BC-893 can be used for the research of cancer and obesity[1][2].

In Vitro

SH-BC-893 starves cancer cells to death by down-regulating cell surface nutrient transporters and blocking lysosomal trafficking events[1].
SH-BC-893 (5 μM; 3 h) protects from ceramide-induced mitochondrial network fragmentation by disrupting intracellular trafficking[2].
SH-BC-893 (8.935 nM; 3 h) protects from ceramide-induced mitochondrial dysfunction[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SH-BC-893 Related Antibodies

Western Blot Analysis[1]

Cell Line: p53 flox/flox MEFs
Concentration: 5 μM
Incubation Time: 3 h
Result: Blocked palmitate-induced recruitment of DRP1 to mitochondria without affecting DRP1 protein levels.

Immunofluorescence[1]

Cell Line: p53 flox/flox MEFs
Concentration: 5 μM
Incubation Time: 3 h
Result: Blocked palmitate-induced recruitment of DRP1 to mitochondria.
In Vivo

SH-BC-893 (oral; 120 mg/kg; single) robustly and acutely blocks ceramide-induced mitochondrial dysfunction, correcting diet-induced obesity and its metabolic sequelae[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6J mice (Male)[1]
Dosage: 120 mg/kg
Administration: Oral; single
Result: Blocked palmitate- and ceramide-induced mitochondrial fission, preserved mitochondrial function, and prevented ER stress.
Normalized mitochondrial morphology in the livers and brains of HFD-fed mice, improved mitochondrial function in white adipose tissue, and corrected aberrant plasma leptin and adiponectin levels.
Restored normal body weight, glucose disposal, and hepatic lipid levels in mice consuming a HFD.
Molecular Weight

325.92

Formula

C19H32ClNO
CAS No.
Appearance

Liquid

Color

Colorless to light yellow

SMILES

OC[C@H]1NCC[C@@H]1C2=CC=C(CCCCCCCC)C=C2.Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (153.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0682 mL 15.3412 mL 30.6824 mL
5 mM 0.6136 mL 3.0682 mL 6.1365 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.67 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (7.67 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.34%

COA (244 KB) HNMR (245 KB) LCMS (90 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.0682 mL 15.3412 mL 30.6824 mL 76.7059 mL
5 mM 0.6136 mL 3.0682 mL 6.1365 mL 15.3412 mL
10 mM 0.3068 mL 1.5341 mL 3.0682 mL 7.6706 mL
15 mM 0.2045 mL 1.0227 mL 2.0455 mL 5.1137 mL
20 mM 0.1534 mL 0.7671 mL 1.5341 mL 3.8353 mL
25 mM 0.1227 mL 0.6136 mL 1.2273 mL 3.0682 mL
30 mM 0.1023 mL 0.5114 mL 1.0227 mL 2.5569 mL
40 mM 0.0767 mL 0.3835 mL 0.7671 mL 1.9176 mL
50 mM 0.0614 mL 0.3068 mL 0.6136 mL 1.5341 mL
60 mM 0.0511 mL 0.2557 mL 0.5114 mL 1.2784 mL
80 mM 0.0384 mL 0.1918 mL 0.3835 mL 0.9588 mL
100 mM 0.0307 mL 0.1534 mL 0.3068 mL 0.7671 mL
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SH-BC-893 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SH-BC-8931841409-92-4Mitochondrial Metabolismoralsphingolipid analogceramide-induced mitochondrial dysfunctiondiet-induced obesityInhibitorinhibitorinhibit

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