SJ995973

Name: SJ995973
Brand: MedChemExpress
SKU: HY-145125
Rating: 5.0 (1 reviews)

Cat. No.: HY-145125 Purity: 98.84%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

SJ995973 (PROTAC) is a uniquely potent degrader of bromodomain and extra-terminal (BET) proteins.

For research use only. We do not sell to patients.

SJ995973 Chemical Structure

CAS No. : 2882065-25-8

Size	Price	Stock	Quantity
1 mg	USD 280	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 850	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 1450	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

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von Hippel-Lindau (VHL) Cereblon MDM2 cIAP1

Biological Activity
Purity & Documentation
References
Customer Review

Description

SJ995973 (PROTAC) is a uniquely potent degrader of bromodomain and extra-terminal (BET) proteins.

Cellular Effect

Cell Line	Type	Value	Description	References
MV4-11	IC₅₀	3 pM Compound: 4c/SJ995973	Antiproliferative activity against human MV4-11 after 3 days by CellTiter-Glo assay Antiproliferative activity against human MV4-11 after 3 days by CellTiter-Glo assay	[PMID: 36274273]
MV4-11	IC₅₀	3 pM Compound: SJ995973; 26	Cytotoxicity against human MV4-11 cells measured after 96 hrs Cytotoxicity against human MV4-11 cells measured after 96 hrs	[PMID: 36202064]

Molecular Weight

730.28

Formula

C₃₇H₄₀ClN₇O₅S

CAS No.

2882065-25-8

Appearance

Solid

Color

Off-white to light yellow

SMILES

CC1=NN=C2[C@@H](N=C(C3=C(N12)SC(C)=C3C)C4=CC=C(C=C4)Cl)CC(NCCCCCNC(COC5=CC=C(C=C5)C6CCC(NC6=O)=O)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (136.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.3693 mL	6.8467 mL	13.6934 mL
5 mM	0.2739 mL	1.3693 mL	2.7387 mL
10 mM	0.1369 mL	0.6847 mL	1.3693 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (3.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.84%

Select Batch:

Data Sheet (269 KB) SDS (252 KB)

COA (245 KB) HNMR (232 KB) RP-HPLC (264 KB) LCMS (108 KB)

Handling Instructions (2659 KB)

References

[1]. Min J, et al. Phenyl-Glutarimides: Alternative Cereblon Binders for the Design of PROTACs. Angew Chem Int Ed Engl. 2021 Dec 13;60(51):26663-26670. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.3693 mL	6.8467 mL	13.6934 mL	34.2334 mL
	5 mM	0.2739 mL	1.3693 mL	2.7387 mL	6.8467 mL
	10 mM	0.1369 mL	0.6847 mL	1.3693 mL	3.4233 mL
	15 mM	0.0913 mL	0.4564 mL	0.9129 mL	2.2822 mL
	20 mM	0.0685 mL	0.3423 mL	0.6847 mL	1.7117 mL
	25 mM	0.0548 mL	0.2739 mL	0.5477 mL	1.3693 mL
	30 mM	0.0456 mL	0.2282 mL	0.4564 mL	1.1411 mL
	40 mM	0.0342 mL	0.1712 mL	0.3423 mL	0.8558 mL
	50 mM	0.0274 mL	0.1369 mL	0.2739 mL	0.6847 mL
	60 mM	0.0228 mL	0.1141 mL	0.2282 mL	0.5706 mL
	80 mM	0.0171 mL	0.0856 mL	0.1712 mL	0.4279 mL
	100 mM	0.0137 mL	0.0685 mL	0.1369 mL	0.3423 mL

SJ995973 Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SJ9959732882065-25-8SJ 995973SJ-995973PROTACsInhibitorinhibitorinhibit

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SJ995973

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SJ995973 Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All PROTACs Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

SJ995973 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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