2. Immunology/Inflammation Anti-infection
  3. Toll-like Receptor (TLR) HIV
  4. Sparstolonin B

Sparstolonin B acts as a selective TLR2 and TLR4 antagonist and selectively blocks TLR2- and TLR4-mediated inflammatory signaling. Sparstolonin B has anti-HIV and anticancer activities.

For research use only. We do not sell to patients.

Sparstolonin B Chemical Structure

Sparstolonin B Chemical Structure

CAS No. : 1259330-61-4

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO USD 121 In-stock
Solid
5 mg USD 110 In-stock
10 mg USD 195 In-stock
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Customer Review

Based on 3 publication(s) in Google Scholar

Sparstolonin B Related Antibodies

Top Publications Citing Use of Products

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Sparstolonin B acts as a selective TLR2 and TLR4 antagonist and selectively blocks TLR2- and TLR4-mediated inflammatory signaling. Sparstolonin B has anti-HIV and anticancer activities[1][2].

IC50 & Target[1][2]

TLR2

 

TLR4

 

HIV-1

 

In Vitro

Sparstolonin B (1-20 μM; 2-4 days) inhibits cell growth and viability of neuroblastoma cells[3].
Sparstolonin B inhibits TLR ligand-induced cytokine expression in mouse macrophages. Sparstolonin B inhibits MyD88 recruitment to TLR4 and TLR2[1].
Sparstolonin B generates reactive oxygen species (ROS) in neuroblastoma cells. Sparstolonin B reduces expression of N-myc in neuroblastoma cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Sparstolonin B Related Antibodies

Cell Viability Assay[3]

Cell Line: SH-SY5Y, IMR-32, NGP, SKNF-1 and SK-N-BE(2) cells
Concentration: 1 µM, 5 µM, 10 µM or 20 µM
Incubation Time: 2-4 days
Result: Effectively and dose-dependently inhibits the viability of all neuroblastoma cell lines after 2 days (SH-SY5Y and IMR-32), 3 days (NGP cells) or 4 days (SKNF-1 and SK-N-BE(2) cells) treatment.
In Vivo

Sparstolonin B (100 μg/mouse; i.p.) suppresses LPS-provoked inflammation in mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 5-6-week-old male C57Bl/6 mice (body weight 18-20 g)[1]
Dosage: 100 μg/mouse
Administration: I.p.
Result: Significantly lower TNFα and IL-1β expression levels in LPS-induced sepsis mouse model.
Molecular Weight

268.22

Formula

C15H8O5
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1OC=C2C3=C(OC4=C2C1=C(O)C=C4)C=CC(O)=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 10 mg/mL (37.28 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.7283 mL 18.6414 mL 37.2828 mL
5 mM 0.7457 mL 3.7283 mL 7.4566 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  0.5% CMC-Na/saline water

    Solubility: 5 mg/mL (18.64 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.54%

SDS (392 KB)

COA (249 KB) HNMR (131 KB) RP-HPLC (247 KB) MS (301 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.7283 mL 18.6414 mL 37.2828 mL 93.2071 mL
5 mM 0.7457 mL 3.7283 mL 7.4566 mL 18.6414 mL
10 mM 0.3728 mL 1.8641 mL 3.7283 mL 9.3207 mL
15 mM 0.2486 mL 1.2428 mL 2.4855 mL 6.2138 mL
20 mM 0.1864 mL 0.9321 mL 1.8641 mL 4.6604 mL
25 mM 0.1491 mL 0.7457 mL 1.4913 mL 3.7283 mL
30 mM 0.1243 mL 0.6214 mL 1.2428 mL 3.1069 mL
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Sparstolonin B Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Sparstolonin B1259330-61-4Toll-like Receptor (TLR)HIVHuman immunodeficiency virusInhibitorinhibitorinhibit

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