Stigmasta-4,22-dien-3-one

Cat. No.: HY-N9596 Purity: 98.82%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

Stigmasta-4,22-dien-3-one is an antitubercular agent. Stigmasta-4,22-dien-3-one shows cytotoxicity against human HT1080 tumoral cell line with an IC₅₀ of 0.3 mM.

For research use only. We do not sell to patients.

Stigmasta-4,22-dien-3-one Chemical Structure

CAS No. : 20817-72-5

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	Get quote
50 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Stigmasta-4,22-dien-3-one:

(22E,24R)-Stigmasta-4,22-dien-3-one Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Stigmasta-4,22-dien-3-one is an antitubercular agent. Stigmasta-4,22-dien-3-one shows cytotoxicity against human HT1080 tumoral cell line with an IC₅₀ of 0.3 mM^[1]^[2].

Molecular Weight

410.67

Formula

C₂₉H₄₆O

CAS No.

20817-72-5

Appearance

Solid

Color

White to off-white

SMILES

C[C@@]12[C@](CC[C@]2([H])[C@H](C)/C=C/[C@@H](CC)C(C)C)([H])[C@@]3([H])[C@@](CC1)([H])[C@@]4(C(CC3)=CC(CC4)=O)C

Structure Classification

Steroids

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

Ethanol : 25 mg/mL (60.88 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4350 mL	12.1752 mL	24.3505 mL
5 mM	0.4870 mL	2.4350 mL	4.8701 mL
10 mM	0.2435 mL	1.2175 mL	2.4350 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.82%

Select Batch:

Data Sheet (271 KB) SDS (251 KB)

COA (249 KB) HNMR (267 KB) RP-HPLC (161 KB) MS (83 KB)

Handling Instructions (2659 KB)

References

[1]. Saludes JP, et al. Antitubercular constituents from the hexane fraction of Morinda citrifolia Linn. (Rubiaceae). Phytother Res. 2002 Nov;16(7):683-5. [Content Brief]

[2]. Sandjo L P, et al. Cytotoxic effect of some pentacyclic triterpenes and hemisynthetic derivatives of stigmasterol. Chemistry of Natural Compounds, 2011, 47(5): 731-734.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol	1 mM	2.4350 mL	12.1752 mL	24.3505 mL	60.8761 mL
	5 mM	0.4870 mL	2.4350 mL	4.8701 mL	12.1752 mL
	10 mM	0.2435 mL	1.2175 mL	2.4350 mL	6.0876 mL
	15 mM	0.1623 mL	0.8117 mL	1.6234 mL	4.0584 mL
	20 mM	0.1218 mL	0.6088 mL	1.2175 mL	3.0438 mL
	25 mM	0.0974 mL	0.4870 mL	0.9740 mL	2.4350 mL
	30 mM	0.0812 mL	0.4058 mL	0.8117 mL	2.0292 mL
	40 mM	0.0609 mL	0.3044 mL	0.6088 mL	1.5219 mL
	50 mM	0.0487 mL	0.2435 mL	0.4870 mL	1.2175 mL
	60 mM	0.0406 mL	0.2029 mL	0.4058 mL	1.0146 mL