Suc-Ala-Ala-Phe-AMC

Cat. No.: HY-137337 Purity: 99.97%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Suc-Ala-Ala-Phe-AMC is a fluorogenic chymotrypsin substrate, can be hydrolyzed by endopeptidase. Suc-Ala-Ala-Phe-AMC has been used in both in vivo assays of the acrosome reaction and in vitro enzyme assays.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Suc-Ala-Ala-Phe-AMC Chemical Structure

CAS No. : 71973-79-0

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 261	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 261	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 70	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 210	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 340	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 650	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1040	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1650	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

Molecular Weight

564.59

Formula

C₂₉H₃₂N₄O₈

CAS No.

71973-79-0

Appearance

Solid

Color

White to off-white

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture and light

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (442.80 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7712 mL	8.8560 mL	17.7120 mL
5 mM	0.3542 mL	1.7712 mL	3.5424 mL
10 mM	0.1771 mL	0.8856 mL	1.7712 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.97%

Select Batch:

Data Sheet (271 KB) SDS (252 KB)

COA (248 KB) HNMR (296 KB) LCMS (91 KB)

Handling Instructions (2659 KB)

References

[1]. Farach HA Jr, et al. Evidence for the involvement of metalloendoproteases in the acrosome reaction in sea urchin sperm. J Biol Chem. 1987 Apr 25;262(12):5483-7. [Content Brief]

[2]. Pinto MR, et al. Chymotrypsin-like enzymes are involved in sperm penetration through the vitelline coat of Ciona intestinalis egg. Mol Reprod Dev. 1990 Aug;26(4):319-23. [Content Brief]

[3]. Mumford RA, et al. A zinc metalloendopeptidase associated with dog pancreatic membranes. J Biol Chem. 1980 Mar 25;255(6):2227-30. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.7712 mL	8.8560 mL	17.7120 mL	44.2799 mL
	5 mM	0.3542 mL	1.7712 mL	3.5424 mL	8.8560 mL
	10 mM	0.1771 mL	0.8856 mL	1.7712 mL	4.4280 mL
	15 mM	0.1181 mL	0.5904 mL	1.1808 mL	2.9520 mL
	20 mM	0.0886 mL	0.4428 mL	0.8856 mL	2.2140 mL
	25 mM	0.0708 mL	0.3542 mL	0.7085 mL	1.7712 mL
	30 mM	0.0590 mL	0.2952 mL	0.5904 mL	1.4760 mL
	40 mM	0.0443 mL	0.2214 mL	0.4428 mL	1.1070 mL
	50 mM	0.0354 mL	0.1771 mL	0.3542 mL	0.8856 mL
	60 mM	0.0295 mL	0.1476 mL	0.2952 mL	0.7380 mL
	80 mM	0.0221 mL	0.1107 mL	0.2214 mL	0.5535 mL
	100 mM	0.0177 mL	0.0886 mL	0.1771 mL	0.4428 mL