T-448 free base 

T-448 free base is a specific, orally active and irreversible inhibitor of lysine-specific demethylase 1 (LSD1, an H3K4 demethylase), with an IC₅₀ of 22 nM. T-448 free base enhances H3K4 methylation in primary cultured rat neurons^[1].

IC50: 22 nM (LSD1)^[1].

T-448 enhances the levels of H3K4 methylation, increased mRNA expression of neural plasticity-related genes including brain derived neurotrophic factor (Bdnf), and ameliorated learning dysfunction^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

T-448 has minimal impact on the LSD1-GFI1B complex and a superior hematological safety profile in mice via the generation of a compact formyl-FAD adduct. T-448 increases brain H3K4 methylation and partially restored learning function in mice with NMDA receptor hypofunction^[1].
T-448 increases H3K4 methylation in the brain without causing hematological side effects even at 100 mg/kg^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

328.43

C₁₇H₂₀N₄OS

1597426-52-2

O=C(NC1=NN=C(C)S1)C2=CC=CC([C@H]3[C@H](NC4CCC4)C3)=C2

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Matsuda S, et al. T-448, a specific inhibitor of LSD1 enzyme activity, improves learning function without causing thrombocytopenia in mice. Neuropsychopharmacology. 2018 Dec 22.  [Content Brief]