1. Metabolic Enzyme/Protease
  2. Endogenous Metabolite Drug Metabolite
  3. Testosterone sulfate (pyridinium)

Testosterone sulfate (pyridinium) 

Cat. No.: HY-113415A Purity: 99.89%
SDS COA Handling Instructions

Testosterone sulfate pyridinium is a Testosterone sulfate with a pyridinium. Testosterone sulfate is the metabolite of Testosterone. Testosterone is the main male sex hormone which determination is useful for assessment of androgen status.

For research use only. We do not sell to patients.

Testosterone sulfate (pyridinium) Chemical Structure

Testosterone sulfate (pyridinium) Chemical Structure

CAS No. : 34991-10-1

Size Price Stock Quantity
5 mg USD 260 In-stock
10 mg USD 380 In-stock
25 mg USD 800 In-stock
50 mg USD 1280 In-stock
100 mg USD 2100 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of Testosterone sulfate (pyridinium):

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Testosterone sulfate (pyridinium)

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Testosterone sulfate pyridinium is a Testosterone sulfate with a pyridinium. Testosterone sulfate is the metabolite of Testosterone. Testosterone is the main male sex hormone which determination is useful for assessment of androgen status[1][2].

IC50 & Target

Human Endogenous Metabolite

 

Molecular Weight

447.59

Formula

C24H33NO5S

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@@]12[C@]3([H])[C@](CCC1=CC(CC2)=O)([H])[C@@]4([H])[C@](CC3)([C@H](CC4)OS(=O)(O)=O)C.C5=CC=NC=C5

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (223.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2342 mL 11.1709 mL 22.3419 mL
5 mM 0.4468 mL 2.2342 mL 4.4684 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.59 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (5.59 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.89%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2342 mL 11.1709 mL 22.3419 mL 55.8547 mL
5 mM 0.4468 mL 2.2342 mL 4.4684 mL 11.1709 mL
10 mM 0.2234 mL 1.1171 mL 2.2342 mL 5.5855 mL
15 mM 0.1489 mL 0.7447 mL 1.4895 mL 3.7236 mL
20 mM 0.1117 mL 0.5585 mL 1.1171 mL 2.7927 mL
25 mM 0.0894 mL 0.4468 mL 0.8937 mL 2.2342 mL
30 mM 0.0745 mL 0.3724 mL 0.7447 mL 1.8618 mL
40 mM 0.0559 mL 0.2793 mL 0.5585 mL 1.3964 mL
50 mM 0.0447 mL 0.2234 mL 0.4468 mL 1.1171 mL
60 mM 0.0372 mL 0.1862 mL 0.3724 mL 0.9309 mL
80 mM 0.0279 mL 0.1396 mL 0.2793 mL 0.6982 mL
100 mM 0.0223 mL 0.1117 mL 0.2234 mL 0.5585 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Testosterone sulfate (pyridinium)
Cat. No.:
HY-113415A
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