2. Membrane Transporter/Ion Channel
  3. EAAT
  4. TFB-TBOA

TFB-TBOA  (Synonyms: CF3-Bza-TBOA)

Cat. No.: HY-107521 Purity: 99.28%
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TFB-TBOA (CF3-Bza-TBOA) is a potent glutamate transporter blocker that potently suppresses the activity of glial transporters. TFB-TBOA shows IC50 values of 22, 17, and 300 nM for glutamate transporters EAAT1, EAAT2, and EAAT3 respectively in an uptake assay using cells transiently expressing EAATs.

For research use only. We do not sell to patients.

TFB-TBOA Chemical Structure

TFB-TBOA Chemical Structure

CAS No. : 480439-73-4

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1 mg USD 220 In-stock
5 mg USD 550 In-stock
10 mg USD 880 In-stock
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Based on 2 publication(s) in Google Scholar

TFB-TBOA Related Antibodies

Top Publications Citing Use of Products

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EAAT1 EAAT2 EAAT3 EAAT4 EAAT5

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Description

TFB-TBOA (CF3-Bza-TBOA) is a potent glutamate transporter blocker that potently suppresses the activity of glial transporters. TFB-TBOA shows IC50 values of 22, 17, and 300 nM for glutamate transporters EAAT1, EAAT2, and EAAT3 respectively in an uptake assay using cells transiently expressing EAATs[1].

IC50 & Target

EAAT1

 

EAAT2

 

EAAT3

 

Cellular Effect
Cell Line Type Value Description References
HEK293 IC50
0.2 μM
Compound: TFB-TBOA
Inhibition of the Excitatory Amino Acid Transporter 1 (EAAT1, SLC1A3) as assessed by a phenotypic impedance-based assay detecting changes in cell morphology by L-glutamate uptake in HEK-293 JumpIN-SLC1A3 cells
Inhibition of the Excitatory Amino Acid Transporter 1 (EAAT1, SLC1A3) as assessed by a phenotypic impedance-based assay detecting changes in cell morphology by L-glutamate uptake in HEK-293 JumpIN-SLC1A3 cells
[PMID: 35677430]
In Vitro

TFB-TBOA (CF3-Bza-TBOA) inhibits synaptically activated transporter currents (STCs) in astrocytes in the stratum radiatum in rat hippocampal slices in a dose-dependent manner with an IC50 of 13 nM, and reduces them to approximately 10% of the control at 100 nM[2].
TFB-TBOA inhibits the Na+i response evoked by 200 µM glutamate in a concentration-dependent manner with IC50 value of 43 nM, as measured on the amplitude of the Na+i response[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

TFB-TBOA Related Antibodies
Molecular Weight

426.34

Formula

C19H17F3N2O6
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CC=C(C=C1)C(F)(F)F)NC2=CC(CO[C@H](C(O)=O)[C@H](N)C(O)=O)=CC=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (117.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3455 mL 11.7277 mL 23.4555 mL
5 mM 0.4691 mL 2.3455 mL 4.6911 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.25 mg/mL (2.93 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.25 mg/mL (2.93 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3455 mL 11.7277 mL 23.4555 mL 58.6386 mL
5 mM 0.4691 mL 2.3455 mL 4.6911 mL 11.7277 mL
10 mM 0.2346 mL 1.1728 mL 2.3455 mL 5.8639 mL
15 mM 0.1564 mL 0.7818 mL 1.5637 mL 3.9092 mL
20 mM 0.1173 mL 0.5864 mL 1.1728 mL 2.9319 mL
25 mM 0.0938 mL 0.4691 mL 0.9382 mL 2.3455 mL
30 mM 0.0782 mL 0.3909 mL 0.7818 mL 1.9546 mL
40 mM 0.0586 mL 0.2932 mL 0.5864 mL 1.4660 mL
50 mM 0.0469 mL 0.2346 mL 0.4691 mL 1.1728 mL
60 mM 0.0391 mL 0.1955 mL 0.3909 mL 0.9773 mL
80 mM 0.0293 mL 0.1466 mL 0.2932 mL 0.7330 mL
100 mM 0.0235 mL 0.1173 mL 0.2346 mL 0.5864 mL
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TFB-TBOA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TFB-TBOA480439-73-4CF3-Bza-TBOAEAATExcitatory amino acid transporter Glutamate transporter| EAAT2EAAT1EAAT3glutamate transportersastrocytesglial transportersInhibitorinhibitorinhibit

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