TPB15

Cat. No.: HY-147670: FAQs
Data Sheet Handling Instructions

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TPB15 is an orally active and potent Hh (Hedgehog) signaling inhibitor. TPB15 markedly induces cell cycle arrest and apoptosis in MDA-MB-468 cells. TPB15 blocks Smo (Smoothened) translocation into the cilia and reduced Smo protein and mRNA expression. TPB15 inhibits the expression of the downstream regulatory factor glioma-associated oncogene 1 (Gli1). TPB15 shows good anti-tumor activity with low toxicity.

For research use only. We do not sell to patients.

TPB15 Chemical Structure

CAS No. : 2170347-69-8

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Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	64.9 μM Compound: TPB15	Antiproliferation activity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferation activity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 32690258]
AN3-CA	IC₅₀	44.5 μM Compound: TPB15	Antiproliferation activity against human AN3CA cells assessed as reduction in cell viability incubated for 72 hr by MTT assay Antiproliferation activity against human AN3CA cells assessed as reduction in cell viability incubated for 72 hr by MTT assay	[PMID: 32690258]
HeLa	IC₅₀	66.3 μM Compound: TPB15	Antiproliferation activity against human HeLa cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferation activity against human HeLa cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 32690258]
MCF-10A	IC₅₀	> 100 μM Compound: TPB15	Cytotoxicity against human MCF10A cells assessed as cell viability incubated for 72 hrs measured by MTT assay Cytotoxicity against human MCF10A cells assessed as cell viability incubated for 72 hrs measured by MTT assay	[PMID: 32690258]
MCF7	IC₅₀	61.5 μM Compound: TPB15	Antiproliferation activity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferation activity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 32690258]
MDA-MB-231	IC₅₀	29.5 μM Compound: TPB15	Antiproliferation activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 72 hrs measured after 4 hrs by MTT assay Antiproliferation activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 72 hrs measured after 4 hrs by MTT assay	[PMID: 32690258]
MDA-MB-468	IC₅₀	28.6 μM Compound: TPB15	Antiproliferation activity against human MDA-MB-468 cells assessed as reduction in cell viability incubated for 72 hr by MTT assay Antiproliferation activity against human MDA-MB-468 cells assessed as reduction in cell viability incubated for 72 hr by MTT assay	[PMID: 32690258]
T47D	IC₅₀	42.3 μM Compound: TPB15	Antiproliferation activity against human T47D cells assessed as reduction in cell viability incubated for 72 hr by MTT assay Antiproliferation activity against human T47D cells assessed as reduction in cell viability incubated for 72 hr by MTT assay	[PMID: 32690258]
U-251	IC₅₀	85.2 μM Compound: TPB15	Antiproliferation activity against human U251 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferation activity against human U251 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 32690258]

Molecular Weight

453.11

Formula

C₁₈H₉Cl₄N₅O

CAS No.

2170347-69-8

SMILES

O=C(C1=C(N=C(C=C1)Cl)Cl)NC2=CC(C3=NN=C4C(Cl)=CC=CN43)=C(C=C2)Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Tian N, et al. Discovery of [1,2,4]triazolo[4,3-a]pyridines as potent Smoothened inhibitors targeting the Hedgehog pathway with improved antitumor activity in vivo. Bioorg Med Chem. 2020 Aug 15;28(16):115584. [Content Brief]

TPB15 Related Classifications

Molarity Calculator
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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass		Concentration		Volume		Molecular Weight *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TPB152170347-69-8TPB 15TPB-15HedgehogSmoGliApoptosisSmoothenedMDA-MB-468 cells,Hedgehog pathwaysmoothened inhibitortriple-negative breast cancergliomaanti-tumor activityInhibitorinhibitorinhibit

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