2. Apoptosis
  3. Apoptosis
  4. USP10-IN-3

USP10-IN-3 (compound D1) is a potent USP10 inhibitor with an IC50 value of 7.2 µM. USP10-IN-3 inhibits cell proliferation. USP10-IN-3 induces apoptosis and cell cycle arrest at S-phase.

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USP10-IN-3 Chemical Structure

USP10-IN-3 Chemical Structure

CAS No. : 3059565-11-3

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Based on 1 publication(s) in Google Scholar

USP10-IN-3 Related Antibodies

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Description

USP10-IN-3 (compound D1) is a potent USP10 inhibitor with an IC50 value of 7.2 µM. USP10-IN-3 inhibits cell proliferation. USP10-IN-3 induces apoptosis and cell cycle arrest at S-phase[1].

IC50 & Target

IC50: 7.2 µM (USP10)[1]
In Vitro

USP10-IN-3 (compound D1) (0-100 µM) inhibits cell proliferation with an IC50 value of 2.3 µM for Huh-7 cells[1].
USP10-IN-3 (compound D1) (0-10 µM; 24 h) induces apoptosis and cell cycle arrest at S-phase in a dose-dependent manner[1].
USP10-IN-3 (compound D1) (0-30 µM; 24 h) decreases the expression of YAP1, p53, p21 in a dose-dependent manner[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

USP10-IN-3 Related Antibodies

Apoptosis Analysis[1]

Cell Line: Huh-7 cells
Concentration: 0, 0.625, 1.25, 2.5, 5, 10 µM
Incubation Time: 24 h
Result: Induced apoptosis in a dose-dependent manner.

Cell Cycle Analysis[1]

Cell Line: Huh-7 cells
Concentration: 0, 0.625, 1.25, 2.5, 5 µM
Incubation Time: 24 h
Result: Induced cell cycle arrest at S-phase.

Western Blot Analysis[1]

Cell Line: Huh-7 cells
Concentration: 0, 0.37, 1.1, 3.3, 10, 30 µM
Incubation Time: 24 h
Result: Decreased the expression of YAP1, p53, p21 in a dose-dependent manner.
Molecular Weight

512.99

Formula

C29H25ClN4O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC1=CC=C2C(C(C3=NC(C4=C(C=CC=C4)C)=NO3)=CN(C2=C1)CC(NCCC5=CC=C(C=C5)Cl)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 5 mg/mL (9.75 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9494 mL 9.7468 mL 19.4936 mL
5 mM 0.3899 mL 1.9494 mL 3.8987 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9494 mL 9.7468 mL 19.4936 mL 48.7339 mL
5 mM 0.3899 mL 1.9494 mL 3.8987 mL 9.7468 mL
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USP10-IN-3 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

USP10-IN-33059565-11-3ApoptosisHuh-7 cellsapoptosisS-phaseanti-proliferationYAP1p53p21Inhibitorinhibitorinhibit

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