USP28-IN-2

Cat. No.: HY-149228: FAQs
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USP28-IN-2 is a USP28 inhibitor (IC₅₀=0.3 μM) with high selectivity over USP2, USP7, USP8, USP9x, UCHL3 and UCHL5. USP28-IN-2 shows cytotoxicity against cancer cells, down-regulates the cellular level of c-Myc through ubiquitin-proteasome system. USP28-IN-2 also decreases the ankyrase-1/2 level in vitro. USP28-IN-2 enhance the sensitivity of colorectal cancer cells to Regorafenib (HY-10331).

For research use only. We do not sell to patients.

USP28-IN-2 Chemical Structure

CAS No. : 2931509-11-2

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USP1 USP2 USP7 USP8 USP30 UCHL1

Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target^[1]

USP28

0.3 μM (IC₅₀)

In Vitro

USP28-IN-2 (compound 9l) (15 μM, 17.5 μM; 3 d) inhibits colony formation of human colorectal cancer cells HCT116 (17.5 μM) and Ls174T (15 μM)^[1].
USP28-IN-2 (20-80 μM; 24 h) down-regulates the level of c-Myc by enhancing its degradation via ubiquitin-proteasome system (UPS)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	Human colorectal cancer cells HCT116 and Ls174T
Concentration:	20 μM, 30 μM, 50 μM, and 60 μM, for Ls174T; 30 μM, 50 μM, 60 μM and 80 μM for HCT116
Incubation Time:	24 h
Result:	Dose-dependently down-regulated the cellular level of c-Myc.

Molecular Weight

475.39

Formula

C₂₃H₂₀Cl₂N₂O₃S

CAS No.

2931509-11-2

SMILES

CS(=O)(C1=CC=C(C(Cl)=C1)C(NC2=CC=C(C(C3=CC=C(C=C3)C4(CC4)N)=C2)Cl)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Zhou D, et al. Structure-based discovery of potent USP28 inhibitors derived from Vismodegib. Eur J Med Chem. 2023 Jun 5;254:115369. [Content Brief]

USP28-IN-2 Related Classifications

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This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

USP28-IN-22931509-11-2DeubiquitinaseDUBsUSP28USP2USP7USP8USP9xUCHL3UCHL5. USP28-IN-2c-Mycubiquitin-proteasome systemankyrase-1/2colorectal cancer cellInhibitorinhibitorinhibit

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