USP7-797 (Synonyms: USP7-IN-7)

Name: USP7-797
Brand: MedChemExpress
SKU: HY-136910
Rating: 4.5 (1 reviews)

Cat. No.: HY-136910 Purity: 97.53%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

USP7-797 (USP7-IN-7) is an orally available, selective USP7 inhibitor (IC₅₀=0.5 nmol/L) with antitumor activity. USP7-797 reduces the level of MDM2, thereby increasing the stability and activity of p53, leading to cell cycle arrest and apoptosis. USP7-797 has low nanomolar cytotoxicity against p53 mutant cancer cell lines, p53 wild-type hematological tumors, and neuroblastoma cell lines.

For research use only. We do not sell to patients.

USP7-797 Chemical Structure

CAS No. : 2413944-70-2

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 1203	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 1203	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 466	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 1072	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 1716	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 3605	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 5768	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 9230	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
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References
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Description

IC₅₀ & Target^[2]

USP7

0.5 nM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
MM1.S	CC₅₀	89 nM Compound: 41	Cytotoxicity against human MM1S cells expressing wild type p53 assessed as reduction in cell viability measured after 5 days by CellTiter-Glo assay Cytotoxicity against human MM1S cells expressing wild type p53 assessed as reduction in cell viability measured after 5 days by CellTiter-Glo assay	[PMID: 32302140]
NCI-H526	CC₅₀	450 nM Compound: 41	Cytotoxicity against human NCI-H526 cells expressing p53 mutant assessed as reduction in cell viability measured after 5 days by CellTiter-Glo assay Cytotoxicity against human NCI-H526 cells expressing p53 mutant assessed as reduction in cell viability measured after 5 days by CellTiter-Glo assay	[PMID: 32302140]
RKO	CC₅₀	13 μM Compound: 41	Cytotoxicity against human RKO cells assessed as reduction in cellular ATP level measured after 5 days by CellTiter-Glo assay Cytotoxicity against human RKO cells assessed as reduction in cellular ATP level measured after 5 days by CellTiter-Glo assay	[PMID: 32302140]
RKO	EC₅₀	25 nM Compound: 41	Inhibition of USP7 in human RKO cells transfected with p53 luciferase reporter vector assessed as increase in p53-dependent luciferase activity measured after 18 hrs by BrightGlo luciferase assay Inhibition of USP7 in human RKO cells transfected with p53 luciferase reporter vector assessed as increase in p53-dependent luciferase activity measured after 18 hrs by BrightGlo luciferase assay	[PMID: 32302140]

In Vitro

USP7-797 (0-1 μM) shows cytotoxicity against p53 wild-type blood cancer cell lines with CC₅₀ values of 0.2, 0.2, 0.4 and 0.1 μM for M07e, OCI-AML5, MOLM13 and MM.IS, respectively^[1].
USP7-797 (0-2 μM) shows cytotoxicity against p53 wild-type neuroblastoma cell lines with CC₅₀ values of 1.9, 0.6 and 0.5 μM for SH-SY5Y, CHP-134 and NB-1, respectively^[1].
USP7-797 (0-25 μM) shows cytotoxicity against p53-mutant cancer cell lines with CC₅₀ values of 0.5, 0.2 and 0.2 μM for H526, LA-N-2 and SK-N-DZ, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

510.05

Formula

C₂₇H₂₈ClN₃O₃S

CAS No.

2413944-70-2

Appearance

Solid

Color

White to off-white

SMILES

O=C(N1CC2=CC3=NC=CC(C4=CC(Cl)=CC(C)=C4C[C@@H]5CNCCO5)=C3S2)C6C(C)(C)C6C1=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 75 mg/mL (147.04 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9606 mL	9.8030 mL	19.6059 mL
5 mM	0.3921 mL	1.9606 mL	3.9212 mL
10 mM	0.1961 mL	0.9803 mL	1.9606 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 3.75 mg/mL (7.35 mM); Clear solution

This protocol yields a clear solution of ≥ 3.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (37.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 97.53%

Select Batch:

Data Sheet (277 KB) SDS (252 KB)

COA (254 KB) HNMR (212 KB) RP-HPLC (406 KB) LCMS (90 KB)

Handling Instructions (2659 KB)

References

[1]. Biannic, Berenger, et al. UBIQUITIN-SPECIFIC-PROCESSING PROTEASE 7 (USP7) MODULATORS AND USES THEREOF. US20200095260. 2019.

[2]. Ohol YM, et al. Novel, Selective Inhibitors of USP7 Uncover Multiple Mechanisms of Antitumor Activity In Vitro and In Vivo. Mol Cancer Ther. 2020 Oct;19(10):1970-1980. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.9606 mL	9.8030 mL	19.6059 mL	49.0148 mL
	5 mM	0.3921 mL	1.9606 mL	3.9212 mL	9.8030 mL
	10 mM	0.1961 mL	0.9803 mL	1.9606 mL	4.9015 mL
	15 mM	0.1307 mL	0.6535 mL	1.3071 mL	3.2677 mL
	20 mM	0.0980 mL	0.4901 mL	0.9803 mL	2.4507 mL
	25 mM	0.0784 mL	0.3921 mL	0.7842 mL	1.9606 mL
	30 mM	0.0654 mL	0.3268 mL	0.6535 mL	1.6338 mL
	40 mM	0.0490 mL	0.2451 mL	0.4901 mL	1.2254 mL
	50 mM	0.0392 mL	0.1961 mL	0.3921 mL	0.9803 mL
	60 mM	0.0327 mL	0.1634 mL	0.3268 mL	0.8169 mL
	80 mM	0.0245 mL	0.1225 mL	0.2451 mL	0.6127 mL
	100 mM	0.0196 mL	0.0980 mL	0.1961 mL	0.4901 mL

USP7-797 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

USP7-7972413944-70-2USP7-IN-7DeubiquitinaseMDM-2/p53DUBsp53blood cancerneuroblastomaH526LA-N-2SK-N-DZSH-SY5YCHP-134NB-1Inhibitorinhibitorinhibit

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USP7-797 (Synonyms: USP7-IN-7)

Customer Review

USP7-797 Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

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View All Deubiquitinase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

USP7-797 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

USP7-797 Related Classifications

Keywords:

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