2. Cell Cycle/DNA Damage Apoptosis
  3. Deubiquitinase MDM-2/p53
  4. USP7-797

USP7-797  (Synonyms: USP7-IN-7)

Cat. No.: HY-136910 Purity: 97.53%
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USP7-797 (USP7-IN-7) is an orally available, selective USP7 inhibitor (IC50=0.5 nmol/L) with antitumor activity. USP7-797 reduces the level of MDM2, thereby increasing the stability and activity of p53, leading to cell cycle arrest and apoptosis. USP7-797 has low nanomolar cytotoxicity against p53 mutant cancer cell lines, p53 wild-type hematological tumors, and neuroblastoma cell lines.

For research use only. We do not sell to patients.

USP7-797 Chemical Structure

USP7-797 Chemical Structure

CAS No. : 2413944-70-2

Size Price Stock Quantity
1 mg USD 466 In-stock
5 mg USD 1072 In-stock
10 mg USD 1716 In-stock
25 mg USD 3605 In-stock
50 mg USD 5768 In-stock
100 mg USD 9230 In-stock
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This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

USP7-797 Related Antibodies

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USP1 USP2 USP7 USP8 USP30 UCHL1

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Description

USP7-797 (USP7-IN-7) is an orally available, selective USP7 inhibitor (IC50=0.5 nmol/L) with antitumor activity. USP7-797 reduces the level of MDM2, thereby increasing the stability and activity of p53, leading to cell cycle arrest and apoptosis. USP7-797 has low nanomolar cytotoxicity against p53 mutant cancer cell lines, p53 wild-type hematological tumors, and neuroblastoma cell lines[1][2].

IC50 & Target[2]

USP7

0.5 nM (IC50)

In Vitro

USP7-797 (0-1 μM) shows cytotoxicity against p53 wild-type blood cancer cell lines with CC50 values of 0.2, 0.2, 0.4 and 0.1 μM for M07e, OCI-AML5, MOLM13 and MM.IS, respectively[1].
USP7-797 (0-2 μM) shows cytotoxicity against p53 wild-type neuroblastoma cell lines with CC50 values of 1.9, 0.6 and 0.5 μM for SH-SY5Y, CHP-134 and NB-1, respectively[1].
USP7-797 (0-25 μM) shows cytotoxicity against p53-mutant cancer cell lines with CC50 values of 0.5, 0.2 and 0.2 μM for H526, LA-N-2 and SK-N-DZ, respectively[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

USP7-797 Related Antibodies
Molecular Weight

510.05

Formula

C27H28ClN3O3S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(N1CC2=CC3=NC=CC(C4=CC(Cl)=CC(C)=C4C[C@@H]5CNCCO5)=C3S2)C6C(C)(C)C6C1=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 75 mg/mL (147.04 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9606 mL 9.8030 mL 19.6059 mL
5 mM 0.3921 mL 1.9606 mL 3.9212 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 3.75 mg/mL (7.35 mM); Clear solution

    This protocol yields a clear solution of ≥ 3.75 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (37.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9606 mL 9.8030 mL 19.6059 mL 49.0148 mL
5 mM 0.3921 mL 1.9606 mL 3.9212 mL 9.8030 mL
10 mM 0.1961 mL 0.9803 mL 1.9606 mL 4.9015 mL
15 mM 0.1307 mL 0.6535 mL 1.3071 mL 3.2677 mL
20 mM 0.0980 mL 0.4901 mL 0.9803 mL 2.4507 mL
25 mM 0.0784 mL 0.3921 mL 0.7842 mL 1.9606 mL
30 mM 0.0654 mL 0.3268 mL 0.6535 mL 1.6338 mL
40 mM 0.0490 mL 0.2451 mL 0.4901 mL 1.2254 mL
50 mM 0.0392 mL 0.1961 mL 0.3921 mL 0.9803 mL
60 mM 0.0327 mL 0.1634 mL 0.3268 mL 0.8169 mL
80 mM 0.0245 mL 0.1225 mL 0.2451 mL 0.6127 mL
100 mM 0.0196 mL 0.0980 mL 0.1961 mL 0.4901 mL
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USP7-797 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

USP7-7972413944-70-2USP7-IN-7DeubiquitinaseMDM-2/p53DUBsp53blood cancerneuroblastomaH526LA-N-2SK-N-DZSH-SY5YCHP-134NB-1Inhibitorinhibitorinhibit

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