2. Apoptosis NF-κB Metabolic Enzyme/Protease Immunology/Inflammation
  3. Apoptosis Reactive Oxygen Species
  4. Verrucarin A

Verrucarin A  (Synonyms: Muconomycin A)

Cat. No.: HY-107426 Purity: 99.89%
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Verrucarin A (Muconomycin A), a Type D macrocyclic mycotoxin derived from the pathogen fungus Myrothecium verrucaria, is an inhibitor of protein synthesis. Verrucarin A inhibits growth of leukemia cell lines and activates caspases and apoptosis and inflammatory signaling in macrophages. Verrucarin A effectively increased the phosphorylation of p38 MAPK and diminished the phosphorylation of ERK/Akt. Verrucarin A caused cell cycle deregulation through the induction of p21 and p53.

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Verrucarin A Chemical Structure

Verrucarin A Chemical Structure

CAS No. : 3148-09-2

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Based on 1 publication(s) in Google Scholar

Verrucarin A Related Antibodies

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Description

Verrucarin A (Muconomycin A), a Type D macrocyclic mycotoxin derived from the pathogen fungus Myrothecium verrucaria, is an inhibitor of protein synthesis. Verrucarin A inhibits growth of leukemia cell lines and activates caspases and apoptosis and inflammatory signaling in macrophages. Verrucarin A effectively increased the phosphorylation of p38 MAPK and diminished the phosphorylation of ERK/Akt. Verrucarin A caused cell cycle deregulation through the induction of p21 and p53[1][2].

Cellular Effect
Cell Line Type Value Description References
HL-60 IC50
0.2 ng/mL
Compound: 3
Cytotoxicity against human HL60 cells after 72 hrs by WST8 assay
Cytotoxicity against human HL60 cells after 72 hrs by WST8 assay
[PMID: 11277768]
K562 IC50
0.003 μM
Compound: 14
Cytotoxicity against human K562 cells by disk diffusion soft agar colony formation assay
Cytotoxicity against human K562 cells by disk diffusion soft agar colony formation assay
[PMID: 31117520]
L1210 IC50
0.35 ng/mL
Compound: 3
Cytotoxicity against mouse L1210 cells after 72 hrs by WST8 assay
Cytotoxicity against mouse L1210 cells after 72 hrs by WST8 assay
[PMID: 11277768]
SW 1116 IC50
0.0018 μM
Compound: 14
Cytotoxicity against human SW1116 cells by disk diffusion soft agar colony formation assay
Cytotoxicity against human SW1116 cells by disk diffusion soft agar colony formation assay
[PMID: 31117520]
In Vitro

Verrucarin A (0-0.6 μM/ml; 24-48 hours)-induces time- and dose-dependent growth inhibition in MCF-7 cells[1].
Verrucarin A increases the levels of reactive oxygen species (ROS), and subsequently induces mitochondrial membrane potential (Δψm) loss, leading to the increase of Bax/Bcl-2 ratio, cytochrome c release, caspase activation, PARP degradation, and apoptosis[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Verrucarin A Related Antibodies

Cell Viability Assay[1]

Cell Line: MCF-7 cells
Concentration: 0-0.6 μM/ml
Incubation Time: 24 and 48 hours
Result: Growth of MCF-7 cells is significantly inhibited in a dose- and time-dependent manner, with the IC50s of 0.41 and 0.29 μM/ml for 24- and 48-h treatment periods, respectively.
Molecular Weight

502.55

Formula

C27H34O9
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

C[C@@]12[C@]3(CO3)[C@@]4([H])C[C@@]1([H])OC(/C=C/C=C/C(OCC[C@@H](C)[C@H](O)C(OC[C@]25[C@](C=C(C)CC5)([H])O4)=O)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 10 mg/mL (19.90 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9899 mL 9.9493 mL 19.8985 mL
5 mM 0.3980 mL 1.9899 mL 3.9797 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation

Purity: 99.89%

SDS (420 KB)

COA (254 KB) RP-HPLC (1071 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9899 mL 9.9493 mL 19.8985 mL 49.7463 mL
5 mM 0.3980 mL 1.9899 mL 3.9797 mL 9.9493 mL
10 mM 0.1990 mL 0.9949 mL 1.9899 mL 4.9746 mL
15 mM 0.1327 mL 0.6633 mL 1.3266 mL 3.3164 mL
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Verrucarin A Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Verrucarin A3148-09-2Muconomycin AApoptosisReactive Oxygen SpeciesmitochondrialmembranedegradationinflammatoryphosphorylationmacrophagesInhibitorinhibitorinhibit

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