2. GPCR/G Protein
  3. Adenosine Receptor
  4. VUF-5574

VUF-5574 is a selective antagonist of adenosine A3 receptor with a Ki of 4.03 nM for the recombinant human receptor.

For research use only. We do not sell to patients.

VUF-5574 Chemical Structure

VUF-5574 Chemical Structure

CAS No. : 280570-45-8

Size Price Stock Quantity
1 mg USD 32 In-stock
5 mg USD 115 In-stock
10 mg USD 185 In-stock
25 mg USD 388 In-stock
50 mg USD 580 In-stock
100 mg   Get quote  
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Based on 1 publication(s) in Google Scholar

VUF-5574 Related Antibodies

Top Publications Citing Use of Products

View All Adenosine Receptor Isoform Specific Products:

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Adenosine A1 receptor (A1R) Adenosine A2A receptor (A2AR) Adenosine A2B receptor (A2BR) Adenosine A3 receptor (A3R)

  • Biological Activity

  • Purity & Documentation

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  • Customer Review

Description

VUF-5574 is a selective antagonist of adenosine A3 receptor with a Ki of 4.03 nM for the recombinant human receptor[1].

IC50 & Target

Recombinant Human Adenosine A3 receptor (A3R)

4.03 nM (Ki)

Molecular Weight

371.39

Formula

C21H17N5O2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=C2C=CC=CC2=NC(C3=CC=CN=C3)=N1)NC4=CC=CC=C4OC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (269.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6926 mL 13.4629 mL 26.9259 mL
5 mM 0.5385 mL 2.6926 mL 5.3852 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (6.73 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6926 mL 13.4629 mL 26.9259 mL 67.3147 mL
5 mM 0.5385 mL 2.6926 mL 5.3852 mL 13.4629 mL
10 mM 0.2693 mL 1.3463 mL 2.6926 mL 6.7315 mL
15 mM 0.1795 mL 0.8975 mL 1.7951 mL 4.4876 mL
20 mM 0.1346 mL 0.6731 mL 1.3463 mL 3.3657 mL
25 mM 0.1077 mL 0.5385 mL 1.0770 mL 2.6926 mL
30 mM 0.0898 mL 0.4488 mL 0.8975 mL 2.2438 mL
40 mM 0.0673 mL 0.3366 mL 0.6731 mL 1.6829 mL
50 mM 0.0539 mL 0.2693 mL 0.5385 mL 1.3463 mL
60 mM 0.0449 mL 0.2244 mL 0.4488 mL 1.1219 mL
80 mM 0.0337 mL 0.1683 mL 0.3366 mL 0.8414 mL
100 mM 0.0269 mL 0.1346 mL 0.2693 mL 0.6731 mL
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VUF-5574 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

VUF-5574280570-45-8VUF5574VUF 5574Adenosine ReceptorP1 receptorInhibitorinhibitorinhibit

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