Improved CaP Nanoparticles for Nucleic Acid and Protein Delivery to Neural Primary Cultures and Stem Cells

Speaker

Hsu-Wen CHAO, PhD
—

• Associate Professor. Department of Physiology, School of Medicine,

  Taipei Medical University

• EMBO Global Investigator Network Award, Europe

• Outstanding Research Award, Taipei Medical University, Taiwan

In this Webinar, You Will Learn:

• The workflow of calcium phosphate (CaP) nanoparticles for the delivery of exogenous materials using a novel two-step transfection method.
• Techniques for delivering both nucleic acids and proteins into primary neurons and neuronal stem cells, without the need for specialized skills or expensive equipment.
• Applications of teleofection in neurobiological research to advance the understanding of neural processes.

About this Webinar:

Delivering exogenous materials into primary neurons and neural stem cells (NSCs) has long been a challenge in neurobiology due to complex protocols, unreliable reproducibility, high immunogenicity, and cytotoxicity.

This webinar introduces teleofection, a cutting-edge two-step process to enhance the formation of biocompatible CaP nanoparticles. Teleofection facilitates the delivery of nucleic acid and protein into primary neurons and NSCs, reducing the need for specialized skills and equipment. Teleofection can easily fine-tune transfection efficiency by adjusting the incubation time and nanoparticle quantity, suitable for various experimental requirements. Teleofection is versatile technique, allowing for the delivery of various cargos into the same cell culture, whether simultaneously or sequentially. This flexibility can be invaluable for long-term studies, such as monitoring neural development and synapse plasticity. Moreover, teleofection ensures consistent and robust expression of the delivered genes, which is essential for molecular and biochemical studies. Teleofection stands out as a significant advancement in neurobiology, offering a user-friendly, cost-effective, and reproducible method that could transform our understanding of brain function and development.

A recording of the webinar will be shared to all registrants.
Participants can request a webinar certificate by contacting Marketing@medchemexpress.com

About Hsu-Wen CHAO, PhD

Hsu-Wen CHAO is a distinguished biologist with a focus on the interplay among the neuroscience, circadian clock and hepatology. He transitioned from neuroscience to chronobiology during his time at Kyoto University. With six years of postdoctoral experience, he developed his technical skills and built professional network at Academia Sinica and various institutions in Japan. In December 2016, Dr Chao was appointed to an independent Assistant Professor at Taipei Medical University (TMU), where he established his own laboratory. His research integrates disciplines such as molecular biology, genetics, oncology, and nanotechnology to address complex biological questions and develop innovative solutions. His team’s long-term focus is on unraveling the mechanisms that govern genome content switch within polyploid tissues (such as liver, brain and small intestine) and the complex relationship between environmental stresses and genome content integrity, with profound implications for human health.

Recent Publications

[1] A transcriptomic Dataset of Liver tissues from Global and Liver Specific Bmal1 Knockout Mice. Scientific Data. 2025 Feb. 13;18 (6):4822-4839. [Content Brief]

[2] Improved CaP nanoparticles transforming nucleic acid and protein delivery to neural primary culture and stem cells. ACS NANO. 2024 Feb. 13;18 (6):4822-4839. [Content Brief]

[3] NR1D1 activation alleviates inflammatory response through inhibition of IL-6 expression in bovine endometrial epithelial cells. International Journal of Biological Macromolecules. 2024 Nov. [Content Brief]

[4] Identification of BMAL1-Regulated circadian genes in mouse liver and their potential association with hepatocellular carcinoma: Gys2 and Upp2 as promising candidates. Biochemical and Biophysical Research Comms. 2024 Feb. (696), 149422. [Content Brief]

[5] From polyploidization to polyploidy reversal, the role in normal and disease states. Trends in Genetics. 2022 Oct 38(10):991-995. [Content Brief]

[6] A common epigenetic mechanism across different cellular origins underlies systemic immune dysregulation in autism. Molecular Psychiatry, 2022 May 02. [Content Brief]

[7] CPEB3-downregulated Nr3c1 mRNA translation confers resilience to developing posttraumatic stress disorder-like behavior in fear-conditioned mice. Neuropsychopharmacology, 2021 May 03, 46, p1669–1679 [Content Brief]

[8] Circadian clock gene BMAL1 controls testosterone production by regulating steroidogenesis-related gene transcription in goat Leydig cells. Journal of Cellular Physiology, 2021 Feb 17, 236 (9), p6706-6725 [Content Brief]