2. Metabolic Enzyme/Protease
  3. Ser/Thr Protease
  4. WNK-IN-1

WNK-IN-1 (compound 7) is a ATP noncompetitive With-No-Lysine (WNK) kinase inhibitor (IC50=95 nM). WNK-IN-1 can be used to regulate cardiovascular homeostasis.

For research use only. We do not sell to patients.

WNK-IN-1 Chemical Structure

WNK-IN-1 Chemical Structure

CAS No. : 2125724-72-1

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1 mg USD 465 In-stock
2 mg USD 698 In-stock
5 mg USD 964 In-stock
10 mg USD 1253 In-stock
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Based on 1 publication(s) in Google Scholar

WNK-IN-1 Related Antibodies

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Description

WNK-IN-1 (compound 7) is a ATP noncompetitive With-No-Lysine (WNK) kinase inhibitor (IC50=95 nM). WNK-IN-1 can be used to regulate cardiovascular homeostasis[1].

IC50 & Target

With-No-Lysine (WNK) kinase[1]
Molecular Weight

399.53

Formula

C26H29N3O
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC(CNCC1=CN(C2=C1C=CC=C2)C3=CC(C4=CC=CC(OC)=C4)=NC=C3C)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (250.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5029 mL 12.5147 mL 25.0294 mL
5 mM 0.5006 mL 2.5029 mL 5.0059 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.5 mg/mL (6.26 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.5 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: 2.5 mg/mL (6.26 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.5029 mL 12.5147 mL 25.0294 mL 62.5735 mL
5 mM 0.5006 mL 2.5029 mL 5.0059 mL 12.5147 mL
10 mM 0.2503 mL 1.2515 mL 2.5029 mL 6.2574 mL
15 mM 0.1669 mL 0.8343 mL 1.6686 mL 4.1716 mL
20 mM 0.1251 mL 0.6257 mL 1.2515 mL 3.1287 mL
25 mM 0.1001 mL 0.5006 mL 1.0012 mL 2.5029 mL
30 mM 0.0834 mL 0.4172 mL 0.8343 mL 2.0858 mL
40 mM 0.0626 mL 0.3129 mL 0.6257 mL 1.5643 mL
50 mM 0.0501 mL 0.2503 mL 0.5006 mL 1.2515 mL
60 mM 0.0417 mL 0.2086 mL 0.4172 mL 1.0429 mL
80 mM 0.0313 mL 0.1564 mL 0.3129 mL 0.7822 mL
100 mM 0.0250 mL 0.1251 mL 0.2503 mL 0.6257 mL
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WNK-IN-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

WNK-IN-12125724-72-1Ser/Thr ProteaseSerine proteasesSerine endopeptidasesThreonine proteasesInhibitorinhibitorinhibit

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