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  3. Fluorescent Dye
  4. WST-3

WST-3 is a water-soluble tetrazolium dye. In the presence of an electron mediator, WST-3 is reduced to WST-3 formazan by NADH and displays an absorption maxima of 433 nM.

For research use only. We do not sell to patients.

WST-3 Chemical Structure

WST-3 Chemical Structure

CAS No. : 515111-36-1

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5 mg USD 35 In-stock
10 mg USD 55 In-stock
25 mg USD 110 In-stock
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Based on 1 publication(s) in Google Scholar

WST-3 Related Antibodies

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Description

WST-3 is a water-soluble tetrazolium dye. In the presence of an electron mediator, WST-3 is reduced to WST-3 formazan by NADH and displays an absorption maxima of 433 nM[1].

Molecular Weight

696.34

Formula

C19H10IN6NaO10S2
CAS No.
Appearance

Solid

Color

Light yellow to green yellow

SMILES

IC1=CC=C(N2N=C(C3=CC=C(S(=O)(O[Na])=O)C=C3S(=O)([O-])=O)N=[N+]2C4=CC=C([N+]([O-])=O)C=C4[N+]([O-])=O)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (143.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4361 mL 7.1804 mL 14.3608 mL
5 mM 0.2872 mL 1.4361 mL 2.8722 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (3.59 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4361 mL 7.1804 mL 14.3608 mL 35.9020 mL
5 mM 0.2872 mL 1.4361 mL 2.8722 mL 7.1804 mL
10 mM 0.1436 mL 0.7180 mL 1.4361 mL 3.5902 mL
15 mM 0.0957 mL 0.4787 mL 0.9574 mL 2.3935 mL
20 mM 0.0718 mL 0.3590 mL 0.7180 mL 1.7951 mL
25 mM 0.0574 mL 0.2872 mL 0.5744 mL 1.4361 mL
30 mM 0.0479 mL 0.2393 mL 0.4787 mL 1.1967 mL
40 mM 0.0359 mL 0.1795 mL 0.3590 mL 0.8976 mL
50 mM 0.0287 mL 0.1436 mL 0.2872 mL 0.7180 mL
60 mM 0.0239 mL 0.1197 mL 0.2393 mL 0.5984 mL
80 mM 0.0180 mL 0.0898 mL 0.1795 mL 0.4488 mL
100 mM 0.0144 mL 0.0718 mL 0.1436 mL 0.3590 mL
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WST-3 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

WST-3515111-36-1WST3WST 3Fluorescent DyeInhibitorinhibitorinhibit

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