2. Metabolic Enzyme/Protease Neuronal Signaling GPCR/G Protein
  3. Aminopeptidase mGluR
  4. β-Spaglumic acid

β-Spaglumic acid  (Synonyms: β-NAAG; β-N-Acetylaspartylglutamic acid)

Cat. No.: HY-130553 Purity: 99.97%
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β-Spaglumic acid (β-NAAG) is a competitive NAAG peptidase inhibitor (Ki=1 µM) that protects spinal cord neurons from excitotoxicity and hypoxic damage. β-Spaglumic acid is also a selective mGluR3 antagonist (mGluR3 receptor functions to regulate activity-dependent synaptic potentiation in the hippocampus). β-Spaglumic acid can be used in neuroprotection-related studies.

For research use only. We do not sell to patients.

β-Spaglumic acid Chemical Structure

β-Spaglumic acid Chemical Structure

CAS No. : 4910-46-7

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Based on 1 publication(s) in Google Scholar

β-Spaglumic acid Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE β-Spaglumic acid

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

β-Spaglumic acid (β-NAAG) is a competitive NAAG peptidase inhibitor (Ki=1 µM) that protects spinal cord neurons from excitotoxicity and hypoxic damage. β-Spaglumic acid is also a selective mGluR3 antagonist (mGluR3 receptor functions to regulate activity-dependent synaptic potentiation in the hippocampus). β-Spaglumic acid can be used in neuroprotection-related studies[1][2].

IC50 & Target

NAAG peptidase, mGluR3[1][2].
In Vitro

β-Spaglumic acid (63-1000 µM; 2 h) protects against NMDA-induced injury of spinal cord cells in a dose-dependent manner[1].
β-Spaglumic acid (0-1000 µM; 2 h) protects spinal cord cells against hypoxia[1].
β-Spaglumic acid (500 µM) significantly reduces intraneuronal free Ca2+ responses upon neuronal exposure to 25 µM NMDA[1].
β-Spaglumic acid (100 µM; 7 min) antagonizes mGluR3 in cerebellar granule cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

β-Spaglumic acid Related Antibodies

Cell Viability Assay[1]

Cell Line: Spinal cord cells (NMDA-induced) (from spinal cords removed from prenatal day 15 Sprague-Dawley rat fetuses)
Concentration: 63-1000 µM
Incubation Time: 2 h
Result: Led to a significant attenuation of NMDA toxicity at a concentration of 63 µM and completely blocked NMDA toxicity with 500 and 1000 µM concentrations.
Apparently minimized the basal loss of cell viability associated with experimental handling of the cells (e.g. serum removal, media changes).

Cell Viability Assay[1]

Cell Line: Spinal cord cells (hypoxic-induced)
Concentration: 0-1000 µM
Incubation Time: 2 h
Result: Provided 75% protection during hypoxia when at 8 µM and completely eliminated hypoxia-induced loss of viability (107.4-114.4% protection, respectively) when at 63-1000 µM.

Cell Viability Assay[2]

Cell Line: Cerebellar granule cells (expressing group I-III mGluRs)
Concentration: 100 µM
Incubation Time: 7 min
Result: Blocked NAAG inhibition of forskolin-stimulated cAMP formation via mGluR3.
Molecular Weight

304.25

Formula

C11H16N2O8
CAS No.
Appearance

Oil

Color

Colorless to off-white

SMILES

O=C(O)CC[C@@H](C(O)=O)NC(C[C@@H](C(O)=O)NC(C)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : ≥ 100 mg/mL (328.68 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2868 mL 16.4339 mL 32.8677 mL
5 mM 0.6574 mL 3.2868 mL 6.5735 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 3.2868 mL 16.4339 mL 32.8677 mL 82.1693 mL
5 mM 0.6574 mL 3.2868 mL 6.5735 mL 16.4339 mL
10 mM 0.3287 mL 1.6434 mL 3.2868 mL 8.2169 mL
15 mM 0.2191 mL 1.0956 mL 2.1912 mL 5.4780 mL
20 mM 0.1643 mL 0.8217 mL 1.6434 mL 4.1085 mL
25 mM 0.1315 mL 0.6574 mL 1.3147 mL 3.2868 mL
30 mM 0.1096 mL 0.5478 mL 1.0956 mL 2.7390 mL
40 mM 0.0822 mL 0.4108 mL 0.8217 mL 2.0542 mL
50 mM 0.0657 mL 0.3287 mL 0.6574 mL 1.6434 mL
60 mM 0.0548 mL 0.2739 mL 0.5478 mL 1.3695 mL
80 mM 0.0411 mL 0.2054 mL 0.4108 mL 1.0271 mL
100 mM 0.0329 mL 0.1643 mL 0.3287 mL 0.8217 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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β-Spaglumic acid Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

β-Spaglumic acid4910-46-7β-NAAG β-N-Acetylaspartylglutamic acidAminopeptidasemGluRMetabotropic glutamate receptorsneuroprotectionmGluR3spinal cord cellscerebellar granule cellsInhibitorinhibitorinhibit

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