2’3’-c-di-AM(PS)2 (Rp,Rp) ammonium salt (Synonyms: ADU-S100 ammonium salt; MIW815 ammonium salt; ML RR-S2 CDA ammonium salt)

Name: 2’3’-c-di-AM(PS)2 (Rp,Rp) ammonium salt
Brand: MedChemExpress
SKU: HY-12885B
Rating: 5.0 (54 reviews)

Cat. No.: HY-12885B Purity: 99.60%: FAQs
Data Sheet SDS COA Handling Instructions

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2’3’-c-di-AM(PS)2 (Rp,Rp) ammonium salt (ADU-S100 ammonium salt), an activator of stimulator of interferon genes (STING), leads to potent and systemic tumor regression and immunity.

For research use only. We do not sell to patients.

2’3’-c-di-AM(PS)2 (Rp,Rp) ammonium salt Chemical Structure

CAS No. : 1638750-96-5

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 1028	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 1028	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 195	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 645	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 1150	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 2400	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 4200	In-stock	Estimated Time of Arrival: December 31
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Based on 54 publication(s) in Google Scholar

Other Forms of 2’3’-c-di-AM(PS)2 (Rp,Rp) ammonium salt:

2’3’-c-di-AM(PS)2 (Rp,Rp) In-stock
2’3’-c-di-AM(PS)2 (Rp,Rp) enantiomer ammonium salt Get quote
2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt Get quote

53 Publications Citing Use of MCE 2’3’-c-di-AM(PS)2 (Rp,Rp) ammonium salt

RT-PCR

: 2’3’-c-di-AM(PS)2 (Rp,Rp) ammonium salt purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Mar 13;14(1):1390. [Abstract]; ADU-S100 (10 μM; 48 h; labeled as STING) enhances type I interferon gene expression in human dendritic cells (DCs).

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Description

2’3’-c-di-AM(PS)2 (Rp,Rp) ammonium salt (ADU-S100 ammonium salt), an activator of stimulator of interferon genes (STING), leads to potent and systemic tumor regression and immunity^[1].

IC₅₀ & Target

STING^[1]

In Vitro

2’3’-c-di-AM(PS)2 (Rp,Rp) ammonium salt has several features that improve both stability and lipophilicity, promoting significantly increased STING signaling as compared to endogenous and pathogen-derived cyclic dinucleotides (CDNs)^[1].
2’3’-c-di-AM(PS)2 (Rp,Rp) shows enhanced type I IFN production over CDA in THP-1 human monocytes. In contrast, the dithio, mixed-linkage cyclic dinucleotide (CDN) derivatives (ML RR-CDA, ML RR-S2 CDG, and ML RR-S2 cGAMP) potently activate all five hSTING alleles, including the refractory hSTING^REF and hSTING^Q alleles. 2’3’-c-di-AM(PS)2 (Rp,Rp) induces the highest expression of IFN-β and the pro-inflammatory cytokines TNF-α, IL-6, and MCP-1 on a molar equivalent basis, as compared to endogenous ML cGAMP and the TLR3 agonist poly I:C. 2’3’-c-di-AM(PS)2 (Rp,Rp) is also found to induce aggregation of STING and induce phosphorylation of TBK1 and IRF3 in mouse bone marrow macrophage (BMM). 2’3’-c-di-AM(PS)2 (Rp,Rp) induces significantly higher levels of IFN-α when compared to ML cGAMP^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

2’3’-c-di-AM(PS)2 (Rp,Rp) shows higher anti-tumor control than the endogenous ML cGAMP. A dose response of the 2’3’-c-di-AM(PS)2 (Rp,Rp) compound is performed in B16 tumor-bearing mice, which identifies an optimal antitumor dose level that also elicites maximum tumor antigen-specific CD8⁺ T cell responses, and improves long-term survival to 50%^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

724.60

Formula

C₂₀H₃₀N₁₂O₁₀P₂S₂

CAS No.

1638750-96-5

Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1[C@@H](O[P@@](OC[C@H]2O[C@@H](N3C(N=CN=C4N)=C4N=C3)[C@H](O)[C@@H]2O5)(S)=O)[C@H](N6C(N=CN=C7N)=C7N=C6)O[C@@H]1CO[P@]5(S)=O.N.N

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (138.01 mM; ultrasonic and warming and heat to 60°C)

DMSO : 15 mg/mL (20.70 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Methanol : 5 mg/mL (6.90 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.3801 mL	6.9004 mL	13.8007 mL
5 mM	0.2760 mL	1.3801 mL	2.7601 mL
10 mM	0.1380 mL	0.6900 mL	1.3801 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 30 mg/mL (41.40 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.89%

Select Batch:

Data Sheet (286 KB) SDS (393 KB)

COA (254 KB) HNMR (279 KB) CNMR (227 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Corrales L, et al. Direct Activation of STING in the Tumor Microenvironment Leads to Potent and Systemic Tumor Regression and Immunity. Cell Rep. 2015 May 19;11(7):1018-30. [Content Brief]

Cell Assay ^[1]	Cryopreserved hPBMCs are thawed and 1×10⁶ cells per well are plated in a 96 well plate in RPMI media supplemented with 10% FBS, 1% non-essential amino acids, 1% penicillin/streptomycin, L-glutamine, 10 mM HEPES buffer, 1 mM Sodium Pyruvate, 0.055 mM β-ME at 37°C with 5% CO₂. Cells are stimulated with 10 μM ADU-S100 or ML cGAMP for 6 hours and supernatants are harvested. Supernatants are diluted 1:2 and assayed for IFN-α protein using Cytometric Bead Array (CBA) Human Flex Set. Data is collected using a FACSVerse cytometer and analyzed by FCAP Array Software^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[1]	Mice^[1] WT C57BL/6 mice are inoculated with 5×10⁴ B16.F10 cells in the left flank (n=8). When tumor volumes are 100 mm³ mice receive three IT doses of either ML RR-S2 CDG (25 μg), ADU-S100 (50 μg), or HBSS as control. WT C57BL/6 mice are inoculated with 5×10⁴ B16.F10 cells in the left flank (n=5). When tumor volumes are 100 mm³ they received three IT doses of ADU-S100 at 5, 25, 50 or 100 μg or HBSS as control. WT C57BL/6 mice are inoculated with 5×10⁴ B16.F10 cells in the left flank (n=8). When tumor volumes are 100 mm³ they receive three IT doses of 100 μg ADU-S100 or HBSS as control. Treatments are administered on days 13, 17 and 20 and tumor measurements are taken twice weekly. Results are shown as percent survival by Log-rank (Mantel-Cox) test (A and C). MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Corrales L, et al. Direct Activation of STING in the Tumor Microenvironment Leads to Potent and Systemic Tumor Regression and Immunity. Cell Rep. 2015 May 19;11(7):1018-30. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Methanol / DMSO / H₂O	1 mM	1.3801 mL	6.9004 mL	13.8007 mL	34.5018 mL
Methanol / DMSO / H₂O	5 mM	0.2760 mL	1.3801 mL	2.7601 mL	6.9004 mL
DMSO / H₂O	10 mM	0.1380 mL	0.6900 mL	1.3801 mL	3.4502 mL
	15 mM	0.0920 mL	0.4600 mL	0.9200 mL	2.3001 mL
	20 mM	0.0690 mL	0.3450 mL	0.6900 mL	1.7251 mL
H₂O	25 mM	0.0552 mL	0.2760 mL	0.5520 mL	1.3801 mL
	30 mM	0.0460 mL	0.2300 mL	0.4600 mL	1.1501 mL
	40 mM	0.0345 mL	0.1725 mL	0.3450 mL	0.8625 mL
	50 mM	0.0276 mL	0.1380 mL	0.2760 mL	0.6900 mL
	60 mM	0.0230 mL	0.1150 mL	0.2300 mL	0.5750 mL
	80 mM	0.0173 mL	0.0863 mL	0.1725 mL	0.4313 mL
	100 mM	0.0138 mL	0.0690 mL	0.1380 mL	0.3450 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

2’3’-c-di-AM(PS)2 (Rp,Rp) ammonium salt Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2’3’-c-di-AM(PS)2 (Rp,Rp)1638750-96-5ADU-S100MIW815ML RR-S2 CDAMIW 815MIW-815STINGStimulator of Interferon GenesTMEM173MITAERISMPYSInhibitorinhibitorinhibit

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2’3’-c-di-AM(PS)2 (Rp,Rp) ammonium salt (Synonyms: ADU-S100 ammonium salt; MIW815 ammonium salt; ML RR-S2 CDA ammonium salt)

Customer Review

Other Forms of 2’3’-c-di-AM(PS)2 (Rp,Rp) ammonium salt:

2’3’-c-di-AM(PS)2 (Rp,Rp) ammonium salt Related Antibodies

53 Publications Citing Use of MCE 2’3’-c-di-AM(PS)2 (Rp,Rp) ammonium salt

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•Related Screening Libraries:

•Related Small Molecules:

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Biological Activity

Protocol

Purity & Documentation

References

Customer Review

2’3’-c-di-AM(PS)2 (Rp,Rp) ammonium salt Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

2’3’-c-di-AM(PS)2 (Rp,Rp) ammonium salt Related Classifications

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