2’3’-c-di-AM(PS)2 (Rp,Rp) (Synonyms: ADU-S100; MIW815; ML RR-S2 CDA)

Cat. No.: HY-12885 Purity: 98.36%: FAQs
Data Sheet SDS COA Handling Instructions

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2’3’-c-di-AM(PS)2 (Rp,Rp) (ADU-S100), an activator of stimulator of interferon genes (STING), leads to potent and systemic tumor regression and immunity.

The free form of the compound is prone to instability, it is advisable to consider the stable salt form (2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt) that retains the same biological activity.

For research use only. We do not sell to patients.

2’3’-c-di-AM(PS)2 (Rp,Rp) Chemical Structure

CAS No. : 1638241-89-0

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Other In-stock Forms of 2’3’-c-di-AM(PS)2 (Rp,Rp):

2’3’-c-di-AM(PS)2 (Rp,Rp) ammonium salt

Other Forms of 2’3’-c-di-AM(PS)2 (Rp,Rp):

2’3’-c-di-AM(PS)2 (Rp,Rp) ammonium salt In-stock
2’3’-c-di-AM(PS)2 (Rp,Rp) enantiomer ammonium salt Get quote
2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt Get quote

53 Publications Citing Use of MCE 2’3’-c-di-AM(PS)2 (Rp,Rp)

RT-PCR

: 2’3’-c-di-AM(PS)2 (Rp,Rp) purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Mar 13;14(1):1390. [Abstract]; ADU-S100 (10 μM; 48 h; labeled as STING) enhances type I interferon gene expression in human dendritic cells (DCs).

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Description

2’3’-c-di-AM(PS)2 (Rp,Rp) (ADU-S100), an activator of stimulator of interferon genes (STING), leads to potent and systemic tumor regression and immunity^[1].

IC₅₀ & Target

STING^[1]

In Vitro

2’3’-c-di-AM(PS)2 (Rp,Rp) is unstable in its free base form. 2’3’-c-di-AM(PS)2 (Rp,Rp) ammonium salt (HY-12885B) improves both stability and lipophilicity, promoting significantly increased STING signaling as compared to endogenous and pathogen-derived cyclic dinucleotides (CDNs)^[1].
2’3’-c-di-AM(PS)2 (Rp,Rp) shows enhanced type I IFN production over CDA in THP-1 human monocytes. In contrast, the dithio, mixed-linkage CDN derivatives (ML RR-CDA, ML RR-S2 CDG, and ML RR-S2 cGAMP) potently activate all five hSTING alleles, including the refractory hSTING^REF and hSTING^Q alleles. 2’3’-c-di-AM(PS)2 (Rp,Rp) induces the highest expression of IFN-β and the pro-inflammatory cytokines TNF-α, IL-6, and MCP-1 on a molar equivalent basis, as compared to endogenous ML cGAMP and the TLR3 agonist poly I:C. 2’3’-c-di-AM(PS)2 (Rp,Rp) is also found to induce aggregation of STING and induce phosphorylation of TBK1 and IRF3 in mouse bone marrow macrophage (BMM). 2’3’-c-di-AM(PS)2 (Rp,Rp) induces significantly higher levels of IFN-α when compared to ML cGAMP^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

2’3’-c-di-AM(PS)2 (Rp,Rp) shows higher anti-tumor control than the endogenous ML cGAMP. A dose response of the 2’3’-c-di-AM(PS)2 (Rp,Rp) compound is performed in B16 tumor-bearing mice, which identifies an optimal antitumor dose level that also elicites maximum tumor antigen-specific CD8⁺ T cell responses, and improves long-term survival to 50%^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

690.54

Formula

C₂₀H₂₄N₁₀O₁₀P₂S₂

CAS No.

1638241-89-0

Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1[C@@H](O[P@@](OC[C@H]2O[C@@H](N3C(N=CN=C4N)=C4N=C3)[C@H](O)[C@@H]2O5)(S)=O)[C@H](N6C(N=CN=C7N)=C7N=C6)O[C@@H]1CO[P@]5(S)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

H₂O : 20 mg/mL (28.96 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.4481 mL	7.2407 mL	14.4814 mL
5 mM	0.2896 mL	1.4481 mL	2.8963 mL
10 mM	0.1448 mL	0.7241 mL	1.4481 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

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Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.85%

Select Batch:

Data Sheet (282 KB) SDS (393 KB)

COA (257 KB) HNMR (487 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

References

[1]. Corrales L, et al. Direct Activation of STING in the Tumor Microenvironment Leads to Potent and Systemic Tumor Regression and Immunity. Cell Rep. 2015 May 19;11(7):1018-30. [Content Brief]

Cell Assay ^[1]	Cryopreserved hPBMCs are thawed and 1×10⁶ cells per well are plated in a 96 well plate in RPMI media. Cells are stimulated with 10 μM ADU-S100 or ML cGAMP for 6 hours and supernatants are harvested. Supernatants are diluted 1:2 and assayed for IFN-α protein using Cytometric Bead Array (CBA) Human Flex Set. Data is collected using a FACSVerse cytometer and analyzed by FCAP Array Software^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[1]	Mice^[1] WT C57BL/6 mice are inoculated with 5×10⁴ B16.F10 cells in the left flank (n=8). When tumor volumes are 100 mm³ mice receive three IT doses of either ML RR-S2 CDG (25 μg), ADU-S100 (50 μg), or HBSS as control. WT C57BL/6 mice are inoculated with 5×10⁴ B16.F10 cells in the left flank (n=5). When tumor volumes are 100 mm³ they received three IT doses of ADU-S100 at 5, 25, 50 or 100 μg or HBSS as control. WT C57BL/6 mice are inoculated with 5×10⁴ B16.F10 cells in the left flank (n=8). When tumor volumes are 100 mm³ they receive three IT doses of 100 μg ADU-S100 or HBSS as control. Treatments are administered on days 13, 17 and 20 and tumor measurements are taken twice weekly. Results are shown as percent survival by Log-rank (Mantel-Cox) test (A and C)^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Corrales L, et al. Direct Activation of STING in the Tumor Microenvironment Leads to Potent and Systemic Tumor Regression and Immunity. Cell Rep. 2015 May 19;11(7):1018-30. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	1.4481 mL	7.2407 mL	14.4814 mL	36.2036 mL
	5 mM	0.2896 mL	1.4481 mL	2.8963 mL	7.2407 mL
	10 mM	0.1448 mL	0.7241 mL	1.4481 mL	3.6204 mL
	15 mM	0.0965 mL	0.4827 mL	0.9654 mL	2.4136 mL
	20 mM	0.0724 mL	0.3620 mL	0.7241 mL	1.8102 mL
	25 mM	0.0579 mL	0.2896 mL	0.5793 mL	1.4481 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

2’3’-c-di-AM(PS)2 (Rp,Rp) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2’3’-c-di-AM(PS)2 (Rp,Rp)1638241-89-0ADU-S100 MIW815 ML RR-S2 CDAMIW815MIW 815MIW-815STINGStimulator of Interferon GenesTMEM173MITAERISMPYSInhibitorinhibitorinhibit

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