2,4-Diacetylphloroglucinol

Name: 2,4-Diacetylphloroglucinol
Brand: MedChemExpress
SKU: HY-118448
Rating: 4.5 (1 reviews)

Cat. No.: HY-118448 Purity: 98.97%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

2,4-Diacetylphloroglucinol, produced by some isolates of the beneficial bacterium Pseudomonas fluorescens, is a potent antibiotic. 2,4-Diacetylphloroglucinol is active against numerous organisms, including plants, fungi, viruses, bacteria, and nematodes.

For research use only. We do not sell to patients.

2,4-Diacetylphloroglucinol Chemical Structure

CAS No. : 2161-86-6

Size	Price	Stock	Quantity
5 mg	USD 78	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 125	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
LLC-PK1	IC₅₀	11 μg/mL Compound: 4	Cytotoxicity against LLC-PK1 cells by neutral red assay Cytotoxicity against LLC-PK1 cells by neutral red assay	[PMID: 17070063]
Vero	IC₅₀	12 μg/mL Compound: 4	Cytotoxicity against Vero cells by neutral red assay Cytotoxicity against Vero cells by neutral red assay	[PMID: 17070063]

Molecular Weight

210.18

Formula

C₁₀H₁₀O₅

CAS No.

2161-86-6

Appearance

Solid

Color

Off-white to light yellow

SMILES

OC1=C(C(O)=C(C(O)=C1)C(C)=O)C(C)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (475.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.7578 mL	23.7891 mL	47.5783 mL
5 mM	0.9516 mL	4.7578 mL	9.5157 mL
10 mM	0.4758 mL	2.3789 mL	4.7578 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (11.89 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (11.89 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (11.89 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.97%

Select Batch:

Data Sheet (275 KB) SDS (393 KB)

COA (254 KB) HNMR (247 KB) LCMS (96 KB) Elemental Analysis Report (112 KB)

Handling Instructions (2659 KB)

References

[1]. Susan L F Meyer, et al. Toxicity of 2,4-diacetylphloroglucinol (DAPG) to plant-parasitic and bacterial-feeding nematodes. J Nematol. 2009 Dec;41(4):274-80. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.7578 mL	23.7891 mL	47.5783 mL	118.9457 mL
	5 mM	0.9516 mL	4.7578 mL	9.5157 mL	23.7891 mL
	10 mM	0.4758 mL	2.3789 mL	4.7578 mL	11.8946 mL
	15 mM	0.3172 mL	1.5859 mL	3.1719 mL	7.9297 mL
	20 mM	0.2379 mL	1.1895 mL	2.3789 mL	5.9473 mL
	25 mM	0.1903 mL	0.9516 mL	1.9031 mL	4.7578 mL
	30 mM	0.1586 mL	0.7930 mL	1.5859 mL	3.9649 mL
	40 mM	0.1189 mL	0.5947 mL	1.1895 mL	2.9736 mL
	50 mM	0.0952 mL	0.4758 mL	0.9516 mL	2.3789 mL
	60 mM	0.0793 mL	0.3965 mL	0.7930 mL	1.9824 mL
	80 mM	0.0595 mL	0.2974 mL	0.5947 mL	1.4868 mL
	100 mM	0.0476 mL	0.2379 mL	0.4758 mL	1.1895 mL