2'-C-Methyladenosine

Name: 2'-C-Methyladenosine
Brand: MedChemExpress
SKU: HY-125371
Rating: 5.0 (2 reviews)

Cat. No.: HY-125371 Purity: 99.63%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

2'-C-Methyladenosine is an inhibitor of hepatitis C virus (HCV) replication. 2'-C-Methyladenosine inhibits HCV replicon and NS5B-catalyzed RNA synthesis with IC₅₀ values of 0.3μM and 1.9 μM, respectively. 2'-C-Methyladenosine also potently inhibits LRV1 in Leishmania guyanensis (Lgy) and Leishmania braziliensis.

For research use only. We do not sell to patients.

2'-C-Methyladenosine Chemical Structure

CAS No. : 15397-12-3

Size	Stock	Quantity

Free Sample (0.1 - 0.5 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

2 Publications Citing Use of MCE 2'-C-Methyladenosine

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

IC50: 0.3μM (HCV replicon); 1.9 μM (NS5B)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
A549	CC₅₀	> 50 μM Compound: 2	Cytotoxicity against human A549 cells assessed as intracellular ATP level by Celltiter-Glo luminescent assay Cytotoxicity against human A549 cells assessed as intracellular ATP level by Celltiter-Glo luminescent assay	[PMID: 20457821]
A549	EC₅₀	1.12 μM Compound: 2	Antiviral activity against at 0.3 MOI Dengue virus 2 infected in human A549 cells assessed as level of E protein after 48 hrs by ELISA Antiviral activity against at 0.3 MOI Dengue virus 2 infected in human A549 cells assessed as level of E protein after 48 hrs by ELISA	[PMID: 20457821]
Caco-2	GI₅₀	> 100 μM Compound: 2'-Me-Ado	Antitumor activity against human CaCo2 cells after 48 hrs by MTS assay Antitumor activity against human CaCo2 cells after 48 hrs by MTS assay	[PMID: 18588281]
HT-29	GI₅₀	> 100 μM Compound: 2'-Me-Ado	Antitumor activity against human HT29 cells after 48 hrs by MTS assay Antitumor activity against human HT29 cells after 48 hrs by MTS assay	[PMID: 18588281]
Huh-5-2	CC₅₀	> 33 μM Compound: 2'-C-Methyladenosine	Cytotoxicity against human Huh5-2 cells after 3 days by MTT assay Cytotoxicity against human Huh5-2 cells after 3 days by MTT assay	[PMID: 18625766]
Huh-5-2	EC₅₀	0.12 μM Compound: 2'-C-Methyladenosine	Antiviral activity against Hepatitis C virus genotype 1b infected in human Huh5-2 cells assessed as reduction in replicon RNA after 4 days by luciferase assay Antiviral activity against Hepatitis C virus genotype 1b infected in human Huh5-2 cells assessed as reduction in replicon RNA after 4 days by luciferase assay	[PMID: 18625766]
Huh-7	CC₅₀	> 100 μM Compound: 1	Cytotoxicity against Huh7 cells after 24 hrs by MTS assay Cytotoxicity against Huh7 cells after 24 hrs by MTS assay	[PMID: 17521911]
Huh-7	CC₅₀	> 100 μM Compound: 2'CmeA	Cytotoxicity against human Huh7 cells after 3 days by MTT method Cytotoxicity against human Huh7 cells after 3 days by MTT method	[PMID: 18078757]
Huh-7	CC₅₀	> 100 μM Compound: 2'CMeA	Cytotoxicity against human Huh7 cells harboring con1 replicon after 3 days by MTT assay Cytotoxicity against human Huh7 cells harboring con1 replicon after 3 days by MTT assay	[PMID: 17997319]
Huh-7	EC₅₀	> 100 μM Compound: 2	Antiviral activity against HCV genotype1 infected in human Huh7 cells assessed as reduction of viral replication by steady-Glo luciferase assay Antiviral activity against HCV genotype1 infected in human Huh7 cells assessed as reduction of viral replication by steady-Glo luciferase assay	[PMID: 20627592]
Huh-7	EC₅₀	0.2 μM Compound: 2 mA	Antiviral activity against HCV genotype 1b infected in human HuH7 cells after 3 days by renilla luciferase reporter assay Antiviral activity against HCV genotype 1b infected in human HuH7 cells after 3 days by renilla luciferase reporter assay	[PMID: 22100256]
Huh-7	EC₅₀	0.2 μM Compound: 1	Antiviral activity against HCV genotype 1b in Huh7 cells assessed as inhibition of viral RNA replication after 72 hrs by ribonuclease protection assay Antiviral activity against HCV genotype 1b in Huh7 cells assessed as inhibition of viral RNA replication after 72 hrs by ribonuclease protection assay	[PMID: 17521911]
Huh-7	EC₅₀	0.25 μM Compound: 1	Antiviral activity against HCV genotype 1b in Huh7 cells assessed as inhibition of viral RNA replication after 24 hrs by ribonuclease protection assay Antiviral activity against HCV genotype 1b in Huh7 cells assessed as inhibition of viral RNA replication after 24 hrs by ribonuclease protection assay	[PMID: 17521911]
Huh-7	EC₅₀	0.35 μM Compound: 2'-C-Me A	Antiviral activity against HCV genotype 1b infected in human HuH7 cells assessed as cytoprotection after 24 hrs Antiviral activity against HCV genotype 1b infected in human HuH7 cells assessed as cytoprotection after 24 hrs	[PMID: 22578461]
Huh-7	CC₅₀	15 μM Compound: 2	Cytotoxicity against Huh7 cells by MTS assay Cytotoxicity against Huh7 cells by MTS assay	[PMID: 16368235]
Huh-7	CC₅₀	55 μM Compound: 2 mA	Cytotoxicity against human HuH7 cells infected with HCV1b after 3 days by MTS assay Cytotoxicity against human HuH7 cells infected with HCV1b after 3 days by MTS assay	[PMID: 22100256]
Huh-7	CC₅₀	66 μM Compound: 2'-C-Me A	Cytotoxicity against human HuH7 cells after 24 hrs by MTS assay Cytotoxicity against human HuH7 cells after 24 hrs by MTS assay	[PMID: 22578461]
K562	GI₅₀	16 μM Compound: 2'-Me-Ado	Antitumor activity against human K562 cells after 48 hrs by MTS assay Antitumor activity against human K562 cells after 48 hrs by MTS assay	[PMID: 18588281]
MCF7	GI₅₀	> 100 μM Compound: 2'-Me-Ado	Antitumor activity against human MCF7 cells after 48 hrs by MTS assay Antitumor activity against human MCF7 cells after 48 hrs by MTS assay	[PMID: 18588281]

In Vivo

2'-C-Methyladenosine has inhibitory potency for HCV replicon in HB110A cells with an IC₅₀ values of 0.3μM^[1].
2'-C-Methyladenosine inhibits NS5B-catalyzed RNA synthesis with an IC₅₀ values of 1.9 μM^[1].
2′-C-methyladenosine potently inhibits LRV1 in Leishmania guyanensis (Lgy) and Leishmania braziliensis^[2]

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

281.27

Formula

C₁₁H₁₅N₅O₄

CAS No.

15397-12-3

Appearance

Solid

Color

White to off-white

SMILES

OC[C@@H]1[C@@H](O)[C@@](C)(O)[C@H](N2C3=NC=NC(N)=C3N=C2)O1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 31.25 mg/mL (111.10 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.5553 mL	17.7765 mL	35.5530 mL
5 mM	0.7111 mL	3.5553 mL	7.1106 mL
10 mM	0.3555 mL	1.7777 mL	3.5553 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.63%

Select Batch:

Data Sheet (278 KB) SDS (252 KB)

COA (254 KB) HNMR (252 KB) RP-HPLC (244 KB) MS (205 KB)

Handling Instructions (2659 KB)

References

[1]. Steven S Carroll, et al. Inhibition of hepatitis C virus RNA replication by 2'-modified nucleoside analogs. J Biol Chem. 2003 Apr 4;278(14):11979-84. [Content Brief]

[2]. John I Robinson, et al. Concentration of 2'C-methyladenosine triphosphate by Leishmania guyanensis enables specific inhibition of Leishmania RNA virus 1 via its RNA polymerase. J Biol Chem. 2018 Apr 27;293(17):6460-6469. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.5553 mL	17.7765 mL	35.5530 mL	88.8826 mL
	5 mM	0.7111 mL	3.5553 mL	7.1106 mL	17.7765 mL
	10 mM	0.3555 mL	1.7777 mL	3.5553 mL	8.8883 mL
	15 mM	0.2370 mL	1.1851 mL	2.3702 mL	5.9255 mL
	20 mM	0.1778 mL	0.8888 mL	1.7777 mL	4.4441 mL
	25 mM	0.1422 mL	0.7111 mL	1.4221 mL	3.5553 mL
	30 mM	0.1185 mL	0.5926 mL	1.1851 mL	2.9628 mL
	40 mM	0.0889 mL	0.4444 mL	0.8888 mL	2.2221 mL
	50 mM	0.0711 mL	0.3555 mL	0.7111 mL	1.7777 mL
	60 mM	0.0593 mL	0.2963 mL	0.5926 mL	1.4814 mL
	80 mM	0.0444 mL	0.2222 mL	0.4444 mL	1.1110 mL
	100 mM	0.0356 mL	0.1778 mL	0.3555 mL	0.8888 mL