2-Hydroxy-4-(methylthio)butyric acid

Cat. No.: HY-116688 Purity: ≥88.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

2-Hydroxy-4-(methylthio)butyric acid is a dietary essential amino acid which is converted to Methionine (HY-13694) by 2-hydroxy acid dehydrogenase and 2-hydroxy acid oxidase. 2-Hydroxy-4-(methylthio)butyric acid is promising for research of gut disease.

For research use only. We do not sell to patients.

2-Hydroxy-4-(methylthio)butyric acid Chemical Structure

CAS No. : 583-91-5

Size	Price	Stock	Quantity
10 g	USD 25	In-stock	Estimated Time of Arrival: December 31
25 g	USD 45	In-stock	Estimated Time of Arrival: December 31
50 g	USD 60	Get quote	Estimated Time of Arrival: December 31
> 100 g		Get quote

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Biological Activity
Purity & Documentation
References
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Description

Molecular Weight

150.20

Formula

C₅H₁₀O₃S

CAS No.

583-91-5

Appearance

Liquid

Color

Light yellow to brown

SMILES

O=C(C(CCSC)O)O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : ≥ 200 mg/mL (1331.56 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.6578 mL	33.2889 mL	66.5779 mL
5 mM	1.3316 mL	6.6578 mL	13.3156 mL
10 mM	0.6658 mL	3.3289 mL	6.6578 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (33.29 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (33.29 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (33.29 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥88.0%

Select Batch:

Data Sheet (269 KB) SDS (476 KB)

COA (244 KB) HNMR (123 KB)

Handling Instructions (2659 KB)

References

[1]. Fang Z, et al. Methionine metabolism in piglets Fed DL-methionine or its hydroxy analogue was affected by distribution of enzymes oxidizing these sources to keto-methionine. J Agric Food Chem. 2010 Feb 10;58(3):2008-14. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	6.6578 mL	33.2889 mL	66.5779 mL	166.4447 mL
	5 mM	1.3316 mL	6.6578 mL	13.3156 mL	33.2889 mL
	10 mM	0.6658 mL	3.3289 mL	6.6578 mL	16.6445 mL
	15 mM	0.4439 mL	2.2193 mL	4.4385 mL	11.0963 mL
	20 mM	0.3329 mL	1.6644 mL	3.3289 mL	8.3222 mL
	25 mM	0.2663 mL	1.3316 mL	2.6631 mL	6.6578 mL
	30 mM	0.2219 mL	1.1096 mL	2.2193 mL	5.5482 mL
	40 mM	0.1664 mL	0.8322 mL	1.6644 mL	4.1611 mL
	50 mM	0.1332 mL	0.6658 mL	1.3316 mL	3.3289 mL
	60 mM	0.1110 mL	0.5548 mL	1.1096 mL	2.7741 mL
	80 mM	0.0832 mL	0.4161 mL	0.8322 mL	2.0806 mL
	100 mM	0.0666 mL	0.3329 mL	0.6658 mL	1.6644 mL