2-PPA

Name: 2-PPA
Brand: MedChemExpress
SKU: HY-33878
Rating: 4.5 (1 reviews)

Cat. No.: HY-33878 Purity: 98.18%: FAQs
Data Sheet SDS COA Handling Instructions

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2-PPA is a selective inhibitor of TMEM175 (IC₅₀=32 μM) with activity in modulating lysosomal function. Acute inhibition of TMEM175 by 2-PPA increases lysosomal macromolecular catabolism, thereby accelerating macrophage and other digestive processes. 2-PPA can be used in the study of Parkinson's disease.

For research use only. We do not sell to patients.

2-PPA Chemical Structure

CAS No. : 21203-86-1

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 27	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 27	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 25	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
1 g	USD 80	In-stock	Estimated Time of Arrival: December 31
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10 g		Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
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References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	IC₅₀	6.9 μM Compound: 12	Inhibition of human ASIC3 expressed in HEK293 cells assessed as inhibition of acid-evoked current by manual patch-clamp electrophysiology assay Inhibition of human ASIC3 expressed in HEK293 cells assessed as inhibition of acid-evoked current by manual patch-clamp electrophysiology assay	[PMID: 21257308]

Molecular Weight

170.22

Formula

C₁₁H₁₀N₂

CAS No.

21203-86-1

Appearance

Solid

Color

White to off-white

SMILES

NC1=CC(C2=CC=CC=C2)=NC=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (587.49 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.8749 mL	29.3746 mL	58.7492 mL
5 mM	1.1750 mL	5.8749 mL	11.7498 mL
10 mM	0.5875 mL	2.9375 mL	5.8749 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (14.69 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (14.69 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (14.69 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.18%

Select Batch:

Data Sheet (270 KB) SDS (393 KB)

COA (246 KB) HNMR (119 KB) RP-HPLC (234 KB)

Handling Instructions (2659 KB)

References

[1]. SeCheol, et al. "Discovery of Selective Inhibitors for the Lysosomal Parkinson’s Disease Channel TMEM175." Journal of the American Chemical Society (2024). [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.8749 mL	29.3746 mL	58.7492 mL	146.8731 mL
	5 mM	1.1750 mL	5.8749 mL	11.7498 mL	29.3746 mL
	10 mM	0.5875 mL	2.9375 mL	5.8749 mL	14.6873 mL
	15 mM	0.3917 mL	1.9583 mL	3.9166 mL	9.7915 mL
	20 mM	0.2937 mL	1.4687 mL	2.9375 mL	7.3437 mL
	25 mM	0.2350 mL	1.1750 mL	2.3500 mL	5.8749 mL
	30 mM	0.1958 mL	0.9792 mL	1.9583 mL	4.8958 mL
	40 mM	0.1469 mL	0.7344 mL	1.4687 mL	3.6718 mL
	50 mM	0.1175 mL	0.5875 mL	1.1750 mL	2.9375 mL
	60 mM	0.0979 mL	0.4896 mL	0.9792 mL	2.4479 mL
	80 mM	0.0734 mL	0.3672 mL	0.7344 mL	1.8359 mL
	100 mM	0.0587 mL	0.2937 mL	0.5875 mL	1.4687 mL

2-PPA Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2-PPA21203-86-1Potassium ChannelKcsATMEM175inhibitorlysosomemacrophageParkinson's diseaseInhibitorinhibit

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2-PPA

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Complete Stock Solution Preparation Table

2-PPA Related Classifications

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