25-O-Methylalisol A

Name: 25-O-Methylalisol A
Brand: MedChemExpress
SKU: HY-N6993
Rating: 4.5 (1 reviews)

Cat. No.: HY-N6993 Purity: 99.60%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

25-O-Methylalisol A is a protostane triterpenoids isolated from Alisma orientale. The dried rhizomes of the aquatic plant Alisma orientale known as Rhizoma Alismatis is a common traditional Chinese medicine used for diuretic, anti-inflammatory, and hypolipidemic purposes, as well as the treatment of diabetes.

For research use only. We do not sell to patients.

25-O-Methylalisol A Chemical Structure

CAS No. : 155801-00-6

Size	Price	Stock	Quantity
1 mg	USD 85	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 250	In-stock	Estimated Time of Arrival: December 31
10 mg		Get quote
50 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
HepG2	EC₅₀	8.9 μM Compound: 18	Transactivation of FXR (unknown origin) transfected in HepG2 cells co-expressing pBSEP/pGL4.74 incubated for 24 hrs by luciferase reporter gene assay Transactivation of FXR (unknown origin) transfected in HepG2 cells co-expressing pBSEP/pGL4.74 incubated for 24 hrs by luciferase reporter gene assay	[PMID: 31494470]

Molecular Weight

504.74

Formula

C₃₁H₅₂O₅

CAS No.

155801-00-6

Appearance

Solid

Color

White to off-white

SMILES

C[C@@]12[C@]([C@H](CC3=C([C@H](C)C[C@H](O)[C@@H](O)C(C)(C)OC)CC[C@]23C)O)([H])[C@@]4([C@@](C(C)(C(CC4)=O)C)([H])CC1)C

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (99.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9812 mL	9.9061 mL	19.8122 mL
5 mM	0.3962 mL	1.9812 mL	3.9624 mL
10 mM	0.1981 mL	0.9906 mL	1.9812 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (2.48 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (2.48 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.25 mg/mL (2.48 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.60%

Select Batch:

Data Sheet (272 KB) SDS (251 KB)

COA (251 KB) HNMR (137 KB) CNMR (136 KB) RP-HPLC (168 KB) MS (78 KB)

Handling Instructions (2659 KB)

References

[1]. Li HM, et al. Protostane-Type Triterpenoids from Alisma orientale. Chem Biodivers. 2017 Dec;14(12). [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.9812 mL	9.9061 mL	19.8122 mL	49.5305 mL
	5 mM	0.3962 mL	1.9812 mL	3.9624 mL	9.9061 mL
	10 mM	0.1981 mL	0.9906 mL	1.9812 mL	4.9530 mL
	15 mM	0.1321 mL	0.6604 mL	1.3208 mL	3.3020 mL
	20 mM	0.0991 mL	0.4953 mL	0.9906 mL	2.4765 mL
	25 mM	0.0792 mL	0.3962 mL	0.7925 mL	1.9812 mL
	30 mM	0.0660 mL	0.3302 mL	0.6604 mL	1.6510 mL
	40 mM	0.0495 mL	0.2477 mL	0.4953 mL	1.2383 mL
	50 mM	0.0396 mL	0.1981 mL	0.3962 mL	0.9906 mL
	60 mM	0.0330 mL	0.1651 mL	0.3302 mL	0.8255 mL
	80 mM	0.0248 mL	0.1238 mL	0.2477 mL	0.6191 mL