3-(Methylthio)propionic acid (Synonyms: 3-Methylsulfanylpropionic acid)

Name: 3-(Methylthio)propionic acid
Brand: MedChemExpress
SKU: HY-101401
Rating: 4.5 (1 reviews)

Cat. No.: HY-101401 Purity: 98.97%: FAQs
Data Sheet SDS COA Handling Instructions

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3-(Methylthio)propionic acid is an intermediate in the methionine metabolism.

For research use only. We do not sell to patients.

3-(Methylthio)propionic acid Chemical Structure

CAS No. : 646-01-5

Size	Price	Stock	Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 50	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 50	In-stock	Estimated Time of Arrival: December 31
Liquid
100 mg	USD 45	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

3-(Methylthio)propionic acid is an intermediate in the methionine metabolism.

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

Methionine has consistently been shown to be the most toxic amino acid in experiments devised to assess the relative toxicity of dietary amino acids. 3-methylthiopropionate is an intermediate in methionine catabolism in rat and monkey liver in vitro. This pathway appears to account for a major portion of methionine oxidation in vitro^[1]. Cultures of Streptomyces lincolnensis accumulated 3-methylthioacrylic acid in amounts directly related to the concentration of methionine in the medium. The first intermediate in the pathway may be the keto acid, which is then oxidatively decarboxylated to 3-methylthiopropionic acid^[2]. The purified 3-MTPA has antifungal activity in assays using F. oxysporum as a model fungus. Daily measurements of shoot and root length shows severe inhibition of seed germination and root and shoot development at concentrations above 12mg for partially purified extracts^[3]. 3-methylthiopropionic acid ethyl ester possesses potential anticarcinogenic properties by inducing differentiation in well-differentiated colon cancer cells. Treatment of RCM-1 cells for 4 days with 3-methylthiopropionic acid ethyl ester between the doses of 0.25 and 2 mM progressively increases the percent area occupied by duct structures relative to the control, and also induces an increase in the number and the maximum diameter of the ducts in each culture plate^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

120.17

Formula

C₄H₈O₂S

CAS No.

646-01-5

Appearance

Liquid (Density: 1.160 g/cm³)

Color

Colorless to light yellow

SMILES

O=C(O)CCSC

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Microorganisms

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 250 mg/mL (2080.39 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 25 mg/mL (208.04 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	8.3215 mL	41.6077 mL	83.2154 mL
5 mM	1.6643 mL	8.3215 mL	16.6431 mL
10 mM	0.8322 mL	4.1608 mL	8.3215 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (832.15 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.97%

Select Batch:

Data Sheet (271 KB) SDS (416 KB)

COA (243 KB) HNMR (237 KB) LCMS (88 KB)

Handling Instructions (2659 KB)

References

[1]. Steele RD, et al. Identification of 3-methylthiopropionic acid as an intermediate in mammalian methioninemetabolism in vitro. J Biol Chem. 1978 Nov 10;253(21):7844-50. [Content Brief]

[2]. Surette R, et al. Formation of 3-methylthioacrylic acid from methionine by Streptomyces lincolnensis. Isolation of a peroxidase. J Antibiot (Tokyo). 1976 Jun;29(6):646-52. [Content Brief]

[3]. Kim YC, et al. 3-methylthiopropanoic acid produced by Enterobacter intermedium 60-2G inhibits fungal growth and weed seedling development. J Antibiot (Tokyo). 2003 Feb;56(2):177-80. [Content Brief]

[4]. Nakamura Y, et al. 3-Methylthiopropionic acid ethyl ester, isolated from Katsura-uri (Japanese pickling melon, Cucumis melo var. conomon), enhanced differentiation in human colon cancer cells. J Agric Food Chem. 2008 May 14;56(9):2977-84. [Content Brief]

Cell Assay
^[4]

RCM-1 cells are each plated into 35-mm plastic culture plates. Twenty-four hours after plating, cells are treated with 3-methylthiopropionic acid ethyl ester in 2 mL of 10% FBS-RPMI+F12 for 4 days, and are counted by using a hemacytometer, after trypsinization with 0.25% trypsin and 1 mM EDTA^[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Steele RD, et al. Identification of 3-methylthiopropionic acid as an intermediate in mammalian methioninemetabolism in vitro. J Biol Chem. 1978 Nov 10;253(21):7844-50. [Content Brief]

[2]. Surette R, et al. Formation of 3-methylthioacrylic acid from methionine by Streptomyces lincolnensis. Isolation of a peroxidase. J Antibiot (Tokyo). 1976 Jun;29(6):646-52. [Content Brief]

[3]. Kim YC, et al. 3-methylthiopropanoic acid produced by Enterobacter intermedium 60-2G inhibits fungal growth and weed seedling development. J Antibiot (Tokyo). 2003 Feb;56(2):177-80. [Content Brief]

[4]. Nakamura Y, et al. 3-Methylthiopropionic acid ethyl ester, isolated from Katsura-uri (Japanese pickling melon, Cucumis melo var. conomon), enhanced differentiation in human colon cancer cells. J Agric Food Chem. 2008 May 14;56(9):2977-84. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	8.3215 mL	41.6077 mL	83.2154 mL	208.0386 mL
	5 mM	1.6643 mL	8.3215 mL	16.6431 mL	41.6077 mL
	10 mM	0.8322 mL	4.1608 mL	8.3215 mL	20.8039 mL
	15 mM	0.5548 mL	2.7738 mL	5.5477 mL	13.8692 mL
	20 mM	0.4161 mL	2.0804 mL	4.1608 mL	10.4019 mL
	25 mM	0.3329 mL	1.6643 mL	3.3286 mL	8.3215 mL
	30 mM	0.2774 mL	1.3869 mL	2.7738 mL	6.9346 mL
	40 mM	0.2080 mL	1.0402 mL	2.0804 mL	5.2010 mL
	50 mM	0.1664 mL	0.8322 mL	1.6643 mL	4.1608 mL
	60 mM	0.1387 mL	0.6935 mL	1.3869 mL	3.4673 mL
	80 mM	0.1040 mL	0.5201 mL	1.0402 mL	2.6005 mL
	100 mM	0.0832 mL	0.4161 mL	0.8322 mL	2.0804 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

3-(Methylthio)propionic acid Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

3-(Methylthio)propionic acid646-01-53-Methylsulfanylpropionic acidFungalEndogenous MetaboliteInhibitorinhibitorinhibit

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3-(Methylthio)propionic acid (Synonyms: 3-Methylsulfanylpropionic acid)

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3-(Methylthio)propionic acid Related Antibodies

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Complete Stock Solution Preparation Table

3-(Methylthio)propionic acid Related Classifications

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