3-Maleimidobenzoic acid N-hydroxysuccinimide ester

Cat. No.: HY-W021068 Purity: 98.81%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

3-Maleimidobenzoic acid N-hydroxysuccinimide ester is used as asymmetric spacers.

For research use only. We do not sell to patients.

3-Maleimidobenzoic acid N-hydroxysuccinimide ester Chemical Structure

CAS No. : 58626-38-3

Size	Price	Stock	Quantity
Free Sample (0.1 - 0.5 mg)		Apply Now
25 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 130	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Purity & Documentation
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Description

3-Maleimidobenzoic acid N-hydroxysuccinimide ester is used as asymmetric spacers^[1].

Molecular Weight

314.25

Formula

C₁₅H₁₀N₂O₆

CAS No.

58626-38-3

Appearance

Solid

Color

White to off-white

SMILES

O=C(ON1C(CCC1=O)=O)C2=CC=CC(N3C(C=CC3=O)=O)=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (318.22 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1822 mL	15.9109 mL	31.8218 mL
5 mM	0.6364 mL	3.1822 mL	6.3644 mL
10 mM	0.3182 mL	1.5911 mL	3.1822 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.96 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.96 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.81%

Select Batch:

Data Sheet (275 KB) SDS (393 KB)

COA (254 KB) HNMR (258 KB) LCMS (87 KB)

Handling Instructions (2659 KB)

References

[1]. N. V. Komarova, et al. Optimization of silicon dioxide surface functionalization protocol for designing the receptor layer of a biosensor for detecting explosives. Moscow University Chemistry Bulletin volume 71, pages 25-31 (2016).

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.1822 mL	15.9109 mL	31.8218 mL	79.5545 mL
	5 mM	0.6364 mL	3.1822 mL	6.3644 mL	15.9109 mL
	10 mM	0.3182 mL	1.5911 mL	3.1822 mL	7.9554 mL
	15 mM	0.2121 mL	1.0607 mL	2.1215 mL	5.3036 mL
	20 mM	0.1591 mL	0.7955 mL	1.5911 mL	3.9777 mL
	25 mM	0.1273 mL	0.6364 mL	1.2729 mL	3.1822 mL
	30 mM	0.1061 mL	0.5304 mL	1.0607 mL	2.6518 mL
	40 mM	0.0796 mL	0.3978 mL	0.7955 mL	1.9889 mL
	50 mM	0.0636 mL	0.3182 mL	0.6364 mL	1.5911 mL
	60 mM	0.0530 mL	0.2652 mL	0.5304 mL	1.3259 mL
	80 mM	0.0398 mL	0.1989 mL	0.3978 mL	0.9944 mL
	100 mM	0.0318 mL	0.1591 mL	0.3182 mL	0.7955 mL