4-[3-(Trifluoromethyl)diazirin-3-yl] benzoic acid N-hydroxysuccinimide ester

Name: 4-[3-(Trifluoromethyl)diazirin-3-yl] benzoic acid N-hydroxysuccinimide ester
Brand: MedChemExpress
SKU: HY-147116
Rating: 4.5 (1 reviews)

Cat. No.: HY-147116 Purity: 99.06%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

4-[3-(Trifluoromethyl)diazirin-3-yl] benzoic acid N-hydroxysuccinimide ester is a photoactivated bifunctional cross-linker. 4-[3-(Trifluoromethyl)diazirin-3-yl] benzoic acid N-hydroxysuccinimide ester can be used for researching a strategy of rapid and accurate structure generation in support of antigen engineering programs.

For research use only. We do not sell to patients.

4-[3-(Trifluoromethyl)diazirin-3-yl] benzoic acid N-hydroxysuccinimide ester Chemical Structure

CAS No. : 87736-89-8

Size	Price	Stock	Quantity
5 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 70	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Purity & Documentation
References
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Description

Molecular Weight

327.22

Formula

C₁₃H₈F₃N₃O₄

CAS No.

87736-89-8

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(C1=CC=C(C2(C(F)(F)F)N=N2)C=C1)ON3C(CCC3=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (305.60 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.0560 mL	15.2802 mL	30.5605 mL
5 mM	0.6112 mL	3.0560 mL	6.1121 mL
10 mM	0.3056 mL	1.5280 mL	3.0560 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.06%

Select Batch:

Data Sheet (276 KB) SDS (604 KB)

COA (254 KB) HNMR (251 KB) RP-HPLC (79 KB)

Handling Instructions (2659 KB)

References

[1]. Ziemianowicz DS, et al. Photo-Cross-Linking Mass Spectrometry and Integrative Modeling Enables Rapid Screening of Antigen Interactions Involving Bacterial Transferrin Receptors. J Proteome Res. 2019 Mar 1;18(3):934-946. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.0560 mL	15.2802 mL	30.5605 mL	76.4012 mL
	5 mM	0.6112 mL	3.0560 mL	6.1121 mL	15.2802 mL
	10 mM	0.3056 mL	1.5280 mL	3.0560 mL	7.6401 mL
	15 mM	0.2037 mL	1.0187 mL	2.0374 mL	5.0934 mL
	20 mM	0.1528 mL	0.7640 mL	1.5280 mL	3.8201 mL
	25 mM	0.1222 mL	0.6112 mL	1.2224 mL	3.0560 mL
	30 mM	0.1019 mL	0.5093 mL	1.0187 mL	2.5467 mL
	40 mM	0.0764 mL	0.3820 mL	0.7640 mL	1.9100 mL
	50 mM	0.0611 mL	0.3056 mL	0.6112 mL	1.5280 mL
	60 mM	0.0509 mL	0.2547 mL	0.5093 mL	1.2734 mL
	80 mM	0.0382 mL	0.1910 mL	0.3820 mL	0.9550 mL
	100 mM	0.0306 mL	0.1528 mL	0.3056 mL	0.7640 mL