4,4-Dimethyl-2-cyclopenten-1-one (Synonyms: 4,4-Dimethylcyclopent-2-enone)

Cat. No.: HY-W024604 Purity: 99.7%: FAQs
Data Sheet SDS COA Handling Instructions

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4,4-Dimethyl-2-cyclopenten-1-one, a natural compound from Apocyniveneti Folium, displays higher tumor-specific cytotoxicity.

For research use only. We do not sell to patients.

4,4-Dimethyl-2-cyclopenten-1-one Chemical Structure

CAS No. : 22748-16-9

Size	Price	Stock	Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 28	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 28	In-stock	Estimated Time of Arrival: December 31
Liquid
10 mg	USD 25	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Purity & Documentation
References
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Description

4,4-Dimethyl-2-cyclopenten-1-one, a natural compound from Apocyniveneti Folium, displays higher tumor-specific cytotoxicity^[1].

In Vitro

4,4-Dimethyl-2-cyclopenten-1-one selectively kills the tumor cells (HSC-2, HL-60). 4,4-Dimethyl-2-cyclopenten-1-one exhibits CC₅₀ (50% cytotoxic concentration) values of 43.8 μg/mL, 152 μg/mL, 118 μg/mL, 14.0 μg/mL, 177 μg/mL and 123 μg/mL in HSC-2, HSC-3, HSG, HL-60, HGF and normal cells (HPC), respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

110.15

Formula

C₇H₁₀O

CAS No.

22748-16-9

Appearance

Liquid (Density: 0.903 g/cm³)

Color

Colorless to light yellow

SMILES

O=C1C=CC(C)(C)C1

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (907.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	9.0785 mL	45.3926 mL	90.7853 mL
5 mM	1.8157 mL	9.0785 mL	18.1571 mL
10 mM	0.9079 mL	4.5393 mL	9.0785 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (22.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (22.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (22.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.95%

Select Batch:

Data Sheet (272 KB) SDS (406 KB)

COA (248 KB) HNMR (222 KB) LCMS (261 KB)

Handling Instructions (2659 KB)

References

[1]. Tohru Nakayachi, et al. Structure-activity relationships of alpha, beta-unsaturated ketones as assessed by their cytotoxicity against oral tumor cells. Anticancer Res. Mar-Apr 2004;24(2B):737-42. [Content Brief]

[2]. Wei-WeiZhu, et al. Cornstalk liquefaction in methanol/water mixed solvents. Fuel Processing Technology Volume 117, January 2014, Pages 1-7.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	9.0785 mL	45.3926 mL	90.7853 mL	226.9632 mL
	5 mM	1.8157 mL	9.0785 mL	18.1571 mL	45.3926 mL
	10 mM	0.9079 mL	4.5393 mL	9.0785 mL	22.6963 mL
	15 mM	0.6052 mL	3.0262 mL	6.0524 mL	15.1309 mL
	20 mM	0.4539 mL	2.2696 mL	4.5393 mL	11.3482 mL
	25 mM	0.3631 mL	1.8157 mL	3.6314 mL	9.0785 mL
	30 mM	0.3026 mL	1.5131 mL	3.0262 mL	7.5654 mL
	40 mM	0.2270 mL	1.1348 mL	2.2696 mL	5.6741 mL
	50 mM	0.1816 mL	0.9079 mL	1.8157 mL	4.5393 mL
	60 mM	0.1513 mL	0.7565 mL	1.5131 mL	3.7827 mL
	80 mM	0.1135 mL	0.5674 mL	1.1348 mL	2.8370 mL
	100 mM	0.0908 mL	0.4539 mL	0.9079 mL	2.2696 mL

4,4-Dimethyl-2-cyclopenten-1-one Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4,4-Dimethyl-2-cyclopenten-1-one22748-16-94,4-Dimethylcyclopent-2-enoneOthersHSCHPFHL-60lowtoxicityInhibitorinhibitorinhibit

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