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  3. 4-Bromo-2-fluoro-1-iodobenzene

4-Bromo-2-fluoro-1-iodobenzene  (Synonyms: 4-Bromo-2-fluoroiodobenzene)

Cat. No.: HY-42121
COA Handling Instructions

4-Bromo-2-fluoro-1-iodobenzene is a biochemical reagent that can be used as a biological material or organic compound for life science related research.

For research use only. We do not sell to patients.

4-Bromo-2-fluoro-1-iodobenzene Chemical Structure

4-Bromo-2-fluoro-1-iodobenzene Chemical Structure

CAS No. : 105931-73-5

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Description

4-Bromo-2-fluoro-1-iodobenzene is a biochemical reagent that can be used as a biological material or organic compound for life science related research.

Molecular Weight

300.89

Formula

C6H3BrFI

CAS No.
Appearance

Solid

Color

White to yellow

SMILES

IC1=C(F)C=C(Br)C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (332.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3235 mL 16.6174 mL 33.2347 mL
5 mM 0.6647 mL 3.3235 mL 6.6469 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (8.31 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.98%

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3235 mL 16.6174 mL 33.2347 mL 83.0868 mL
5 mM 0.6647 mL 3.3235 mL 6.6469 mL 16.6174 mL
10 mM 0.3323 mL 1.6617 mL 3.3235 mL 8.3087 mL
15 mM 0.2216 mL 1.1078 mL 2.2156 mL 5.5391 mL
20 mM 0.1662 mL 0.8309 mL 1.6617 mL 4.1543 mL
25 mM 0.1329 mL 0.6647 mL 1.3294 mL 3.3235 mL
30 mM 0.1108 mL 0.5539 mL 1.1078 mL 2.7696 mL
40 mM 0.0831 mL 0.4154 mL 0.8309 mL 2.0772 mL
50 mM 0.0665 mL 0.3323 mL 0.6647 mL 1.6617 mL
60 mM 0.0554 mL 0.2770 mL 0.5539 mL 1.3848 mL
80 mM 0.0415 mL 0.2077 mL 0.4154 mL 1.0386 mL
100 mM 0.0332 mL 0.1662 mL 0.3323 mL 0.8309 mL
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4-Bromo-2-fluoro-1-iodobenzene Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
4-Bromo-2-fluoro-1-iodobenzene
Cat. No.:
HY-42121
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