2. Metabolic Enzyme/Protease
  3. Tryptophan Hydroxylase
  4. p-Ethynylphenylalanine hydrochloride

p-Ethynylphenylalanine hydrochloride  (Synonyms: 4-Ethynyl-L-phenylalanine hydrochloride)

Cat. No.: HY-23460A Purity: 99.42%
COA Handling Instructions

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p-Ethynylphenylalanine hydrochloride (4-Ethynyl-L-phenylalanine hydrochloride) is a potent, selective, reversible and competitive inhibitor of tryptophan hydroxylase (TPH), with a Ki of 32.6 μM. p-Ethynylphenylalanine (hydrochloride) is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

For research use only. We do not sell to patients.

p-Ethynylphenylalanine hydrochloride Chemical Structure

p-Ethynylphenylalanine hydrochloride Chemical Structure

CAS No. : 188640-63-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 88 In-stock
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10 mM * 1 mL in DMSO USD 88 In-stock
Solid
50 mg USD 80 In-stock
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200 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

Other Forms of p-Ethynylphenylalanine hydrochloride:

p-Ethynylphenylalanine hydrochloride Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE p-Ethynylphenylalanine hydrochloride

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

p-Ethynylphenylalanine hydrochloride (4-Ethynyl-L-phenylalanine hydrochloride) is a potent, selective, reversible and competitive inhibitor of tryptophan hydroxylase (TPH), with a Ki of 32.6 μM[1]. p-Ethynylphenylalanine (hydrochloride) is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

IC50 & Target

Ki: 32.6 μM (TPH)[1]
In Vitro

p-Ethynylphenylalanine hydrochloride selectively and reversibly inhibits the biosynthesis of serotonin[1].
? p-Ethynylphenylalanine hydrochloride has a low affinity for various recombinant 5-HT receptors (5-HT1, 5-HT2, 5-HT4, 5-HT5, 5-HT6, and 5-HT7)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

p-Ethynylphenylalanine hydrochloride Related Antibodies
In Vivo

p-Ethynylphenylalanine hydrochloride (30 mg/kg; i.p.) decreases in 5-HT and 5-HIAA levels in the rat midbrain but not in tissue[1].
? p-Ethynylphenylalanine hydrochloride does not inhibit the aromatic amino acid decarboxylase[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Sprague-Dawley rats (200 g)[1]
Dosage: 30 mg/kg
Administration: Intraperitoneal injection
Result: Decreased in 5-HT and 5-HIAA levels in the rat midbrain.
Molecular Weight

225.67

Formula

C11H12ClNO2
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

N[C@@H](CC1=CC=C(C#C)C=C1)C(O)=O.[H]Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (221.56 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 12.5 mg/mL (55.39 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.4312 mL 22.1562 mL 44.3125 mL
5 mM 0.8862 mL 4.4312 mL 8.8625 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (11.08 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL (11.08 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.42%

COA (247 KB) HNMR (185 KB) LCMS (111 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 4.4312 mL 22.1562 mL 44.3125 mL 110.7812 mL
5 mM 0.8862 mL 4.4312 mL 8.8625 mL 22.1562 mL
10 mM 0.4431 mL 2.2156 mL 4.4312 mL 11.0781 mL
15 mM 0.2954 mL 1.4771 mL 2.9542 mL 7.3854 mL
20 mM 0.2216 mL 1.1078 mL 2.2156 mL 5.5391 mL
25 mM 0.1772 mL 0.8862 mL 1.7725 mL 4.4312 mL
30 mM 0.1477 mL 0.7385 mL 1.4771 mL 3.6927 mL
40 mM 0.1108 mL 0.5539 mL 1.1078 mL 2.7695 mL
50 mM 0.0886 mL 0.4431 mL 0.8862 mL 2.2156 mL
DMSO 60 mM 0.0739 mL 0.3693 mL 0.7385 mL 1.8464 mL
80 mM 0.0554 mL 0.2770 mL 0.5539 mL 1.3848 mL
100 mM 0.0443 mL 0.2216 mL 0.4431 mL 1.1078 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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p-Ethynylphenylalanine hydrochloride Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

p-Ethynylphenylalanine188640-63-34-Ethynyl-L-phenylalanineTryptophan HydroxylasetryptophanhydroxylaseTPHserotonin5-HT5-HIAAInhibitorinhibitorinhibit

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