2. Metabolic Enzyme/Protease
  3. Phosphatase
  4. 4-Methylumbelliferyl phosphate disodium

4-Methylumbelliferyl phosphate disodium  (Synonyms: 4-MUP disodium; MUP disodium)

Cat. No.: HY-D0994A
Handling Instructions

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4-Methylumbelliferyl phosphate (4-MUP) disodium, an anionic organophosphate, is a acid and alkaline phosphatase fluorogenic substrate. 4-Methylumbelliferyl phosphate disodium is also a nerve agent simulant.

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4-Methylumbelliferyl phosphate disodium Chemical Structure

4-Methylumbelliferyl phosphate disodium Chemical Structure

CAS No. : 22919-26-2

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Other Forms of 4-Methylumbelliferyl phosphate disodium:

4-Methylumbelliferyl phosphate disodium Related Antibodies

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Description

4-Methylumbelliferyl phosphate (4-MUP) disodium, an anionic organophosphate, is a acid and alkaline phosphatase fluorogenic substrate. 4-Methylumbelliferyl phosphate disodium is also a nerve agent simulant[1][2][3].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Serum acid phosphatase assay[1]:
1. Preparation a 100 μL solution containing the following agents: 5.0 μL of serum enzyme, 50 μL of 4-Methylumbelliferyl phosphate (5.0 mM), and 10 μL of 1.0 M buffer at pH 6.0.
2. Add the following agents (with the final concentration) 20 mM Sodium Tartrate (HY-128476), 15 mM Sodium Fluoride (HY-B1766), 3000 mM 2-Mercaptoethanol, 4 mM 1, 10-Phenanthroline (HY-W004544), 6 mM 8-Quinolinol (HY-B1005), 6 mM 8-Hydroxy-5quinolinesulfonic acid, 45 mM EDTA (HY-Y0682) and 6 mM EGTA (HY-D0861).
3. Incubate reaction mixtures for 15 min at 37℃ and terminated by the addition of 2.9 mL of 0.1 M ammonium hydroxide/glycine buffer, pH 10.5.
4. Mix samples thoroughly and determine fluorescence by a fluorometer.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

4-Methylumbelliferyl phosphate disodium Related Antibodies
Molecular Weight

300.11

Formula

C10H7Na2O6P
CAS No.
Emission (Em)

460
Excitation (Ex)

344
SMILES

O=C1C=C(C)C2=CC=C(OP(O[Na])(O[Na])=O)C=C2O1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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4-Methylumbelliferyl phosphate disodium Related Classifications

  • Molarity Calculator

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4-Methylumbelliferyl phosphate disodium22919-26-24-MUP disodiumMUP disodiumPhosphataseInhibitorinhibitorinhibit

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