4-Nitrophenyl butyrate

Name: 4-Nitrophenyl butyrate
Brand: MedChemExpress
SKU: HY-W014449
Rating: 4.5 (1 reviews)

Cat. No.: HY-W014449 Purity: 99.66%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

4-Nitrophenyl butyrate consists of butyric acid chains esterified with 4-nitrophenol groups, thus giving it a yellow color. This compound is commonly used as a substrate in enzyme assays to measure esterase and lipase activity. When these enzymes cleave the ester bond, the nitrophenol group is released and the color changes from yellow to orange. Thus, the rate of color change can be used to determine enzyme activity. In addition, 4-Nitrophenyl butyrate can also be used as organic synthesis reagent and dye intermediate.

For research use only. We do not sell to patients.

4-Nitrophenyl butyrate Chemical Structure

CAS No. : 2635-84-9

Size	Price	Stock	Quantity
1 g	USD 50	In-stock	Estimated Time of Arrival: December 31
5 g	USD 140	In-stock	Estimated Time of Arrival: December 31
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50 g		Get quote

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Based on 1 publication(s) in Google Scholar

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Purity & Documentation
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Description

In Vitro

4-Nitrophenyl butyrate is a biochemical reagent that can be used as a biological material or organic compound for life science related research.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

209.20

Formula

C₁₀H₁₁NO₄

CAS No.

2635-84-9

Appearance

Liquid (Density: 1.19 g/cm³)

Color

Colorless to light yellow

SMILES

CCCC(OC1=CC=C([N+]([O-])=O)C=C1)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (478.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.7801 mL	23.9006 mL	47.8011 mL
5 mM	0.9560 mL	4.7801 mL	9.5602 mL
10 mM	0.4780 mL	2.3901 mL	4.7801 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (11.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (11.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.66%

Select Batch:

Data Sheet (244 KB) SDS (393 KB)

COA (245 KB) HNMR (116 KB) RP-HPLC (237 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.7801 mL	23.9006 mL	47.8011 mL	119.5029 mL
	5 mM	0.9560 mL	4.7801 mL	9.5602 mL	23.9006 mL
	10 mM	0.4780 mL	2.3901 mL	4.7801 mL	11.9503 mL
	15 mM	0.3187 mL	1.5934 mL	3.1867 mL	7.9669 mL
	20 mM	0.2390 mL	1.1950 mL	2.3901 mL	5.9751 mL
	25 mM	0.1912 mL	0.9560 mL	1.9120 mL	4.7801 mL
	30 mM	0.1593 mL	0.7967 mL	1.5934 mL	3.9834 mL
	40 mM	0.1195 mL	0.5975 mL	1.1950 mL	2.9876 mL
	50 mM	0.0956 mL	0.4780 mL	0.9560 mL	2.3901 mL
	60 mM	0.0797 mL	0.3983 mL	0.7967 mL	1.9917 mL
	80 mM	0.0598 mL	0.2988 mL	0.5975 mL	1.4938 mL
	100 mM	0.0478 mL	0.2390 mL	0.4780 mL	1.1950 mL