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  4. 5-Bromo-3-indolyl β-D-galactopyranoside

5-Bromo-3-indolyl β-D-galactopyranoside  (Synonyms: Bluo-Gal)

Cat. No.: HY-137276 Purity: 99.73%
SDS COA Handling Instructions

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5-Bromo-3-indolyl β-D-galactopyranoside (Bluo-Gal) is a chromogenic substrate to detect bacterial β-D-galactosidase activity. 5-Bromo-3-indolyl β-D-galactopyranoside is used both to recognize labelled myofibers, and beta-gal positive organelles inside single myofibers.

For research use only. We do not sell to patients.

5-Bromo-3-indolyl β-D-galactopyranoside Chemical Structure

5-Bromo-3-indolyl β-D-galactopyranoside Chemical Structure

CAS No. : 97753-82-7

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO USD 30 In-stock
Solid
5 mg USD 28 In-stock
10 mg USD 38 In-stock
50 mg USD 61 In-stock
100 mg USD 95 In-stock
250 mg USD 190 In-stock
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Based on 1 publication(s) in Google Scholar

5-Bromo-3-indolyl β-D-galactopyranoside Related Antibodies

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Description

5-Bromo-3-indolyl β-D-galactopyranoside (Bluo-Gal) is a chromogenic substrate to detect bacterial β-D-galactosidase activity. 5-Bromo-3-indolyl β-D-galactopyranoside is used both to recognize labelled myofibers, and beta-gal positive organelles inside single myofibers[1].

Molecular Weight

374.18

Formula

C14H16BrNO6
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

BrC1=CC=C2C(C(O[C@@H]([C@@H]([C@H]3O)O)O[C@@H]([C@@H]3O)CO)=CN2)=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (334.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6725 mL 13.3626 mL 26.7251 mL
5 mM 0.5345 mL 2.6725 mL 5.3450 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.73%

SDS (252 KB)

COA (253 KB) HNMR (253 KB) LCMS (95 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6725 mL 13.3626 mL 26.7251 mL 66.8128 mL
5 mM 0.5345 mL 2.6725 mL 5.3450 mL 13.3626 mL
10 mM 0.2673 mL 1.3363 mL 2.6725 mL 6.6813 mL
15 mM 0.1782 mL 0.8908 mL 1.7817 mL 4.4542 mL
20 mM 0.1336 mL 0.6681 mL 1.3363 mL 3.3406 mL
25 mM 0.1069 mL 0.5345 mL 1.0690 mL 2.6725 mL
30 mM 0.0891 mL 0.4454 mL 0.8908 mL 2.2271 mL
40 mM 0.0668 mL 0.3341 mL 0.6681 mL 1.6703 mL
50 mM 0.0535 mL 0.2673 mL 0.5345 mL 1.3363 mL
60 mM 0.0445 mL 0.2227 mL 0.4454 mL 1.1135 mL
80 mM 0.0334 mL 0.1670 mL 0.3341 mL 0.8352 mL
100 mM 0.0267 mL 0.1336 mL 0.2673 mL 0.6681 mL
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5-Bromo-3-indolyl β-D-galactopyranoside Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5-Bromo-3-indolyl β-D-galactopyranoside97753-82-7Bluo-GalFluorescent Dyechromogenic substratemyofibersβ-D-galactosidaselacZInhibitorinhibitorinhibit

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