5-Dihydrocortisone

Name: 5-Dihydrocortisone
Brand: MedChemExpress
SKU: HY-N8549A
Rating: 4.5 (1 reviews)

Cat. No.: HY-N8549A Purity: ≥99.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

5-Dihydrocortisone is a 5-Dihydrocortisone with mixture of isomers. 5β-Dihydrocortisone is a sterol metabolite of cortisone by 5β-reductase (AKR1D1) in liver

For research use only. We do not sell to patients.

5-Dihydrocortisone Chemical Structure

CAS No. : 28423-48-5

Size	Price	Stock	Quantity
10 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 190	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of 5-Dihydrocortisone:

5β-Dihydrocortisone Get quote

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

5-Dihydrocortisone is a 5-Dihydrocortisone with mixture of isomers. 5β-Dihydrocortisone is a sterol metabolite of cortisone by 5β-reductase (AKR1D1) in liver^[1]

Molecular Weight

362.46

Formula

C₂₁H₃₀O₅

CAS No.

28423-48-5

Appearance

Solid

Color

White to off-white

SMILES

C[C@@]12[C@](CC[C@]2(O)C(CO)=O)([H])[C@@]3([H])[C@@](C(C1)=O)([H])[C@@]4(C(CC(CC4)=O)([H])CC3)C

Structure Classification

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (275.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7589 mL	13.7946 mL	27.5893 mL
5 mM	0.5518 mL	2.7589 mL	5.5179 mL
10 mM	0.2759 mL	1.3795 mL	2.7589 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥99.0%

Select Batch:

Data Sheet (273 KB) SDS (252 KB)

COA (252 KB)

Handling Instructions (2659 KB)

References

[1]. Nikolaos Nikolaou, et al. AKR1D1 regulates glucocorticoid availability and glucocorticoid receptor activation in human hepatoma cells. J Steroid Biochem Mol Biol [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.7589 mL	13.7946 mL	27.5893 mL	68.9731 mL
	5 mM	0.5518 mL	2.7589 mL	5.5179 mL	13.7946 mL
	10 mM	0.2759 mL	1.3795 mL	2.7589 mL	6.8973 mL
	15 mM	0.1839 mL	0.9196 mL	1.8393 mL	4.5982 mL
	20 mM	0.1379 mL	0.6897 mL	1.3795 mL	3.4487 mL
	25 mM	0.1104 mL	0.5518 mL	1.1036 mL	2.7589 mL
	30 mM	0.0920 mL	0.4598 mL	0.9196 mL	2.2991 mL
	40 mM	0.0690 mL	0.3449 mL	0.6897 mL	1.7243 mL
	50 mM	0.0552 mL	0.2759 mL	0.5518 mL	1.3795 mL
	60 mM	0.0460 mL	0.2299 mL	0.4598 mL	1.1496 mL
	80 mM	0.0345 mL	0.1724 mL	0.3449 mL	0.8622 mL
	100 mM	0.0276 mL	0.1379 mL	0.2759 mL	0.6897 mL