5-Fluoro-5'-deoxycytidine

Name: 5-Fluoro-5'-deoxycytidine
Brand: MedChemExpress
SKU: HY-W009538
Rating: 4.5 (1 reviews)

Cat. No.: HY-W009538 Purity: 99.91%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

5-Fluoro-5'-deoxycytidine is a cytidine analog. Cytidine analogs have a mechanism of inhibiting DNA methyltransferases (such as Zebularine, HY-13420), and have potential anti-metabolic and anti-tumor activities.

For research use only. We do not sell to patients.

5-Fluoro-5'-deoxycytidine Chemical Structure

CAS No. : 66335-38-4

Size	Price	Stock	Quantity
Free Sample (0.1 - 0.5 mg)		Apply Now
1 g	USD 30	In-stock	Estimated Time of Arrival: December 31
5 g	USD 70	In-stock	Estimated Time of Arrival: December 31
10 g		Get quote
50 g		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of 5-Fluoro-5'-deoxycytidine:

5-Fluoro-5'-deoxycytidine-d₃ Get quote
5-Fluoro-5'-deoxycytidine-¹³C₅ Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Molecular Weight

245.21

Formula

C₉H₁₂FN₃O₄

CAS No.

66335-38-4

Appearance

Solid

Color

White to off-white

SMILES

O=C(N=C(N)C(F)=C1)N1[C@@H]2O[C@H](C)[C@@H](O)[C@H]2O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (407.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.0781 mL	20.3907 mL	40.7814 mL
5 mM	0.8156 mL	4.0781 mL	8.1563 mL
10 mM	0.4078 mL	2.0391 mL	4.0781 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.20 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.20 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (10.20 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.91%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (252 KB) HNMR (133 KB) RP-HPLC (225 KB) LCMS (257 KB)

Handling Instructions (2659 KB)

References

[1]. Gowher H, et al. Mechanism of inhibition of DNA methyltransferases by cytidine analogs in cancer therapy. Cancer Biol Ther. 2004 Nov;3(11):1062-8. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.0781 mL	20.3907 mL	40.7814 mL	101.9534 mL
	5 mM	0.8156 mL	4.0781 mL	8.1563 mL	20.3907 mL
	10 mM	0.4078 mL	2.0391 mL	4.0781 mL	10.1953 mL
	15 mM	0.2719 mL	1.3594 mL	2.7188 mL	6.7969 mL
	20 mM	0.2039 mL	1.0195 mL	2.0391 mL	5.0977 mL
	25 mM	0.1631 mL	0.8156 mL	1.6313 mL	4.0781 mL
	30 mM	0.1359 mL	0.6797 mL	1.3594 mL	3.3984 mL
	40 mM	0.1020 mL	0.5098 mL	1.0195 mL	2.5488 mL
	50 mM	0.0816 mL	0.4078 mL	0.8156 mL	2.0391 mL
	60 mM	0.0680 mL	0.3398 mL	0.6797 mL	1.6992 mL
	80 mM	0.0510 mL	0.2549 mL	0.5098 mL	1.2744 mL
	100 mM	0.0408 mL	0.2039 mL	0.4078 mL	1.0195 mL