5-Hydroxymethylcytosine (Synonyms: 5hmC)

Cat. No.: HY-W018324 Purity: 98.14%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

5-Hydroxymethylcytosine (5hmC) is an oxidized forms of 5-methylcytosine (5mC) in mammalian DNA. 5-Hydroxymethylcytosine is produced from 5mC in an enzymatic pathway involving three 5mC oxidases, Ten-eleven translocation (TET)1, TET2, and TET3. The conversion of 5mC into 5hmC can be the first step in a pathway leading towards DNA demethylation. 5-Hydroxymethylcytosine is associated with gene transcription and frequently used as a mark to investigate dynamic DNA methylation conversion during mammalian development.

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5-Hydroxymethylcytosine Chemical Structure

CAS No. : 1123-95-1

Size	Price	Stock	Quantity
1 mg	USD 48	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 112	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 168	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 340	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Other Forms of 5-Hydroxymethylcytosine:

5-Hydroxymethylcytosine-¹³C,d₂ Get quote

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Description

Molecular Weight

141.13

Formula

C₅H₇N₃O₂

CAS No.

1123-95-1

Appearance

Solid

Color

Off-white to yellow

SMILES

O=C1N=CC(CO)=C(N1)N

Structure Classification

Others

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 6.25 mg/mL (44.29 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	7.0857 mL	35.4283 mL	70.8567 mL
5 mM	1.4171 mL	7.0857 mL	14.1713 mL
10 mM	0.7086 mL	3.5428 mL	7.0857 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.91%

Select Batch:

Data Sheet (271 KB) SDS (393 KB)

COA (248 KB) RP-HPLC (283 KB)

Handling Instructions (2659 KB)

References

[1]. Gerd P Pfeifer, et al. 5-hydroxymethylcytosine and its potential roles in development and cancer. Epigenetics Chromatin. 2013 May 1;6(1):10. [Content Brief]

[2]. Xiao-Long Cui, et al. A human tissue map of 5-hydroxymethylcytosines exhibits tissue specificity through gene and enhancer modulation. Nat Commun. 2020 Dec 2;11(1):6161. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	7.0857 mL	35.4283 mL	70.8567 mL	177.1416 mL
	5 mM	1.4171 mL	7.0857 mL	14.1713 mL	35.4283 mL
	10 mM	0.7086 mL	3.5428 mL	7.0857 mL	17.7142 mL
	15 mM	0.4724 mL	2.3619 mL	4.7238 mL	11.8094 mL
	20 mM	0.3543 mL	1.7714 mL	3.5428 mL	8.8571 mL
	25 mM	0.2834 mL	1.4171 mL	2.8343 mL	7.0857 mL
	30 mM	0.2362 mL	1.1809 mL	2.3619 mL	5.9047 mL
	40 mM	0.1771 mL	0.8857 mL	1.7714 mL	4.4285 mL

5-Hydroxymethylcytosine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5-Hydroxymethylcytosine1123-95-15hmCEndogenous Metabolite5-methylcytosine5mCmammalian5mC oxidasesTen-eleven translocationTET1TET2TET3DNA demethylationgene transcriptionInhibitorinhibitorinhibit

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